Generation of DNA profiles from fabrics without DNA extraction

A.M.T. Linacre, Vera Pekarek, Yuvaneswari Chandramoulee Swaran, Shanan Tobe

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

DNA profiles can be obtained from fabrics where a person has made direct contact with clothing. A standard approach is to cut out a section of the fabric and then use a commercially available method to extract and isolate the DNA. Alternative methods to isolate DNA include the use of adhesive tape to remove traces of cellular material from the fabric prior to extraction. We report on a process to obtain full DNA profiles using direct amplification from a range of fabrics. The absence of an extraction step both reduces the opportunity for contamination and reduces the loss of DNA during the extraction process, increasing the sensitivity of the process of generating a DNA profile. The process does not require the use of commercially available extraction kits thus reducing the cost of generating a DNA profile from trace amounts of starting material. The results are in part dependent upon the nature of the fabric used to which the DNA has been transferred.
LanguageEnglish
Pages137-141
Number of pages5
JournalForensic Science International: Genetics
Volume4
Issue number2
Early online date1 Sep 2009
DOIs
Publication statusPublished - Feb 2010

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DNA
Clothing
Adhesives
Costs and Cost Analysis

Keywords

  • DNA extraction
  • direct PCR
  • STR
  • nylon
  • cotton
  • polyester

Cite this

Linacre, A.M.T. ; Pekarek, Vera ; Chandramoulee Swaran, Yuvaneswari ; Tobe, Shanan. / Generation of DNA profiles from fabrics without DNA extraction. In: Forensic Science International: Genetics . 2010 ; Vol. 4, No. 2. pp. 137-141.
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Linacre, AMT, Pekarek, V, Chandramoulee Swaran, Y & Tobe, S 2010, 'Generation of DNA profiles from fabrics without DNA extraction' Forensic Science International: Genetics , vol. 4, no. 2, pp. 137-141. https://doi.org/10.1016/j.fsigen.2009.07.006

Generation of DNA profiles from fabrics without DNA extraction. / Linacre, A.M.T.; Pekarek, Vera; Chandramoulee Swaran, Yuvaneswari; Tobe, Shanan.

In: Forensic Science International: Genetics , Vol. 4, No. 2, 02.2010, p. 137-141.

Research output: Contribution to journalArticle

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