Gelatine and gelatine/elastin nanocomposites for vascular grafts: processing and characterisation

Dimitrios Lamprou, P Zhdan, F Labeed, C Lekakou

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This study involves the preparation, microstructural, physical, mechanical, and biological characterization of novel gelatine and gelatine/elastin gels for their use in the tissue engineering of vascular grafts. Gelatine and gelatine/elastin nanocomposite gels were prepared via a sol-gel process, using soluble gelatine. Gelatine was subsequently cross-linked by leaving the gels in 1% glutaraldehyde. The cross-linking time was optimized by assessing the mass loss of the cross-linked gels in water and examining their mechanical properties in dynamic mechanical tests. Atomic force microscopy (AFM) studies revealed elastin nanodomains, homogeneously distributed and embedded in a bed of gelatine nanofibrils in the 30/70 elastin/gelatine gel. It was concluded that the manufactured nanocomposite gels resembled natural arteries in terms of microstructure and stiffness. The biological characterization involved the culture of rat smooth muscle cells (SMCs) on tubular gelatine and gelatine/ elastin nanocomposite gels, and measurements of the scaffold diameter and the cell density as a function of time.
LanguageEnglish
Pages209-226
Number of pages18
JournalJournal of Biomaterials Applications
Volume26
Issue number2
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Elastin
Grafts
Nanocomposites
Gels
Processing
Glutaral
Scaffolds (biology)
Tissue engineering
Cell culture
Scaffolds
Sol-gel process
Muscle
Rats
Atomic force microscopy
Cells
Stiffness
Mechanical properties
Microstructure
Water

Keywords

  • vascular
  • graft
  • gel
  • gelatine
  • composite
  • nanocomposite

Cite this

Lamprou, Dimitrios ; Zhdan, P ; Labeed, F ; Lekakou, C. / Gelatine and gelatine/elastin nanocomposites for vascular grafts : processing and characterisation. In: Journal of Biomaterials Applications. 2011 ; Vol. 26, No. 2. pp. 209-226.
@article{0b8e226abf22418d9d260bcda82ee9a6,
title = "Gelatine and gelatine/elastin nanocomposites for vascular grafts: processing and characterisation",
abstract = "This study involves the preparation, microstructural, physical, mechanical, and biological characterization of novel gelatine and gelatine/elastin gels for their use in the tissue engineering of vascular grafts. Gelatine and gelatine/elastin nanocomposite gels were prepared via a sol-gel process, using soluble gelatine. Gelatine was subsequently cross-linked by leaving the gels in 1{\%} glutaraldehyde. The cross-linking time was optimized by assessing the mass loss of the cross-linked gels in water and examining their mechanical properties in dynamic mechanical tests. Atomic force microscopy (AFM) studies revealed elastin nanodomains, homogeneously distributed and embedded in a bed of gelatine nanofibrils in the 30/70 elastin/gelatine gel. It was concluded that the manufactured nanocomposite gels resembled natural arteries in terms of microstructure and stiffness. The biological characterization involved the culture of rat smooth muscle cells (SMCs) on tubular gelatine and gelatine/ elastin nanocomposite gels, and measurements of the scaffold diameter and the cell density as a function of time.",
keywords = "vascular, graft, gel, gelatine, composite, nanocomposite",
author = "Dimitrios Lamprou and P Zhdan and F Labeed and C Lekakou",
year = "2011",
month = "8",
doi = "10.1177/0885328210364429",
language = "English",
volume = "26",
pages = "209--226",
journal = "Journal of Biomaterials Applications",
issn = "0885-3282",
number = "2",

}

Gelatine and gelatine/elastin nanocomposites for vascular grafts : processing and characterisation. / Lamprou, Dimitrios; Zhdan, P; Labeed, F; Lekakou, C.

In: Journal of Biomaterials Applications, Vol. 26, No. 2, 08.2011, p. 209-226.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gelatine and gelatine/elastin nanocomposites for vascular grafts

T2 - Journal of Biomaterials Applications

AU - Lamprou, Dimitrios

AU - Zhdan, P

AU - Labeed, F

AU - Lekakou, C

PY - 2011/8

Y1 - 2011/8

N2 - This study involves the preparation, microstructural, physical, mechanical, and biological characterization of novel gelatine and gelatine/elastin gels for their use in the tissue engineering of vascular grafts. Gelatine and gelatine/elastin nanocomposite gels were prepared via a sol-gel process, using soluble gelatine. Gelatine was subsequently cross-linked by leaving the gels in 1% glutaraldehyde. The cross-linking time was optimized by assessing the mass loss of the cross-linked gels in water and examining their mechanical properties in dynamic mechanical tests. Atomic force microscopy (AFM) studies revealed elastin nanodomains, homogeneously distributed and embedded in a bed of gelatine nanofibrils in the 30/70 elastin/gelatine gel. It was concluded that the manufactured nanocomposite gels resembled natural arteries in terms of microstructure and stiffness. The biological characterization involved the culture of rat smooth muscle cells (SMCs) on tubular gelatine and gelatine/ elastin nanocomposite gels, and measurements of the scaffold diameter and the cell density as a function of time.

AB - This study involves the preparation, microstructural, physical, mechanical, and biological characterization of novel gelatine and gelatine/elastin gels for their use in the tissue engineering of vascular grafts. Gelatine and gelatine/elastin nanocomposite gels were prepared via a sol-gel process, using soluble gelatine. Gelatine was subsequently cross-linked by leaving the gels in 1% glutaraldehyde. The cross-linking time was optimized by assessing the mass loss of the cross-linked gels in water and examining their mechanical properties in dynamic mechanical tests. Atomic force microscopy (AFM) studies revealed elastin nanodomains, homogeneously distributed and embedded in a bed of gelatine nanofibrils in the 30/70 elastin/gelatine gel. It was concluded that the manufactured nanocomposite gels resembled natural arteries in terms of microstructure and stiffness. The biological characterization involved the culture of rat smooth muscle cells (SMCs) on tubular gelatine and gelatine/ elastin nanocomposite gels, and measurements of the scaffold diameter and the cell density as a function of time.

KW - vascular

KW - graft

KW - gel

KW - gelatine

KW - composite

KW - nanocomposite

U2 - 10.1177/0885328210364429

DO - 10.1177/0885328210364429

M3 - Article

VL - 26

SP - 209

EP - 226

JO - Journal of Biomaterials Applications

JF - Journal of Biomaterials Applications

SN - 0885-3282

IS - 2

ER -