GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex

Paul G. Anastasiades, Andre Marques-Smith, Daniel Lyngholm, Tom Lickiss, Sayda Raffiq, Dennis Kätzel, Gero Miesenböck, Simon J.B. Butt

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

GABAergic interneurons play key roles in cortical circuits, yet little is known about their early connectivity. Here we use glutamate uncaging and a novel optogenetic strategy to track changes in the afferent and efferent synaptic connections of developing neocortical interneuron subtypes. We find that Nkx2-1-derived interneurons possess functional synaptic connections before emerging pyramidal cell networks. Subsequent interneuron circuit maturation is both subtype and layer dependent. Glutamatergic input onto fast spiking (FS), but not somatostatin-positive, non-FS interneurons increases over development. Interneurons of both subtype located in layers (L) 4 and 5b engage in transient circuits that disappear after the somatosensory critical period. These include a pathway mediated by L5b somatostatin-positive interneurons that specifically targets L4 during the first postnatal week. The innervation patterns of immature cortical interneuron circuits are thus neither static nor progressively strengthened but follow a layer-specific choreography of transient connections that differ from those of the adult brain.
LanguageEnglish
Article number10584
Number of pages13
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 4 Feb 2016

Fingerprint

Neocortex
Interneurons
spiking
Networks (circuits)
Somatostatin
glutamates
brain
Glutamic Acid
emerging
Brain
Optogenetics
Pyramidal Cells
cells

Keywords

  • brain development
  • cell fate
  • cell maturation
  • evoked response
  • immunohistochemistry
  • in vitro study
  • interneuron

Cite this

Anastasiades, P. G., Marques-Smith, A., Lyngholm, D., Lickiss, T., Raffiq, S., Kätzel, D., ... Butt, S. J. B. (2016). GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nature Communications, 7, [10584]. https://doi.org/10.1038/ncomms10584
Anastasiades, Paul G. ; Marques-Smith, Andre ; Lyngholm, Daniel ; Lickiss, Tom ; Raffiq, Sayda ; Kätzel, Dennis ; Miesenböck, Gero ; Butt, Simon J.B. / GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. In: Nature Communications. 2016 ; Vol. 7.
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Anastasiades, PG, Marques-Smith, A, Lyngholm, D, Lickiss, T, Raffiq, S, Kätzel, D, Miesenböck, G & Butt, SJB 2016, 'GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex' Nature Communications, vol. 7, 10584. https://doi.org/10.1038/ncomms10584

GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. / Anastasiades, Paul G.; Marques-Smith, Andre; Lyngholm, Daniel; Lickiss, Tom; Raffiq, Sayda; Kätzel, Dennis; Miesenböck, Gero; Butt, Simon J.B.

In: Nature Communications, Vol. 7, 10584, 04.02.2016.

Research output: Contribution to journalArticle

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Anastasiades PG, Marques-Smith A, Lyngholm D, Lickiss T, Raffiq S, Kätzel D et al. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nature Communications. 2016 Feb 4;7. 10584. https://doi.org/10.1038/ncomms10584