Abstract
| Language | English |
|---|---|
| Pages | 697-701 |
| Number of pages | 5 |
| Journal | Biochemical Society Transactions |
| Volume | 37 |
| DOIs | |
| Publication status | Published - 22 Mar 2009 |
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Keywords
- bioanalysis
- functionalized nanoparticle
- immunoassay
- metal surface
- surface-enhanced resonance Raman scattering (SERRS)
- surface plasmon resonance (SPR)
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Functionalized nanoparticles for bioanalysis by SERRS. / Graham, D.; Faulds, Karen; Thompson, David; McKenzie, Fiona; Stokes, Robert J.; Dalton, Colette; Stevenson, Ross; Alexander, J.; Garside, Paul; McFarlane, Emma.
In: Biochemical Society Transactions, Vol. 37, 22.03.2009, p. 697-701.Research output: Contribution to journal › Article
TY - JOUR
T1 - Functionalized nanoparticles for bioanalysis by SERRS
AU - Graham, D.
AU - Faulds, Karen
AU - Thompson, David
AU - McKenzie, Fiona
AU - Stokes, Robert J.
AU - Dalton, Colette
AU - Stevenson, Ross
AU - Alexander, J.
AU - Garside, Paul
AU - McFarlane, Emma
PY - 2009/3/22
Y1 - 2009/3/22
N2 - Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.
AB - Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.
KW - bioanalysis
KW - functionalized nanoparticle
KW - immunoassay
KW - metal surface
KW - surface-enhanced resonance Raman scattering (SERRS)
KW - surface plasmon resonance (SPR)
UR - http://oasys2.confex.com/acs/237nm/techprogram/P1245712.HTM
U2 - 10.1042/BST0370697
DO - 10.1042/BST0370697
M3 - Article
VL - 37
SP - 697
EP - 701
JO - Biochemical Society Transactions
T2 - Biochemical Society Transactions
JF - Biochemical Society Transactions
SN - 0300-5127
ER -