Functionalized nanoparticles for bioanalysis by SERRS

D. Graham, Karen Faulds, David Thompson, Fiona McKenzie, Robert J. Stokes, Colette Dalton, Ross Stevenson, J. Alexander, Paul Garside, Emma McFarlane

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.
LanguageEnglish
Pages697-701
Number of pages5
JournalBiochemical Society Transactions
Volume37
DOIs
Publication statusPublished - 22 Mar 2009

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Raman Spectrum Analysis
Nanoparticles
Raman scattering
Metal Nanoparticles
Molecular recognition
Immunoassay
Silver
Gold
Biomolecules
Biological systems
Spectrum Analysis
Fluorescence
Labels
Switches
Spectroscopy
Antibodies
DNA
Imaging techniques
Molecules
Substrates

Keywords

  • bioanalysis
  • functionalized nanoparticle
  • immunoassay
  • metal surface
  • surface-enhanced resonance Raman scattering (SERRS)
  • surface plasmon resonance (SPR)

Cite this

Graham, D., Faulds, K., Thompson, D., McKenzie, F., Stokes, R. J., Dalton, C., ... McFarlane, E. (2009). Functionalized nanoparticles for bioanalysis by SERRS. Biochemical Society Transactions, 37, 697-701. https://doi.org/10.1042/BST0370697
Graham, D. ; Faulds, Karen ; Thompson, David ; McKenzie, Fiona ; Stokes, Robert J. ; Dalton, Colette ; Stevenson, Ross ; Alexander, J. ; Garside, Paul ; McFarlane, Emma. / Functionalized nanoparticles for bioanalysis by SERRS. In: Biochemical Society Transactions. 2009 ; Vol. 37. pp. 697-701.
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Graham, D, Faulds, K, Thompson, D, McKenzie, F, Stokes, RJ, Dalton, C, Stevenson, R, Alexander, J, Garside, P & McFarlane, E 2009, 'Functionalized nanoparticles for bioanalysis by SERRS' Biochemical Society Transactions, vol. 37, pp. 697-701. https://doi.org/10.1042/BST0370697

Functionalized nanoparticles for bioanalysis by SERRS. / Graham, D.; Faulds, Karen; Thompson, David; McKenzie, Fiona; Stokes, Robert J.; Dalton, Colette; Stevenson, Ross; Alexander, J.; Garside, Paul; McFarlane, Emma.

In: Biochemical Society Transactions, Vol. 37, 22.03.2009, p. 697-701.

Research output: Contribution to journalArticle

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