Functionalized nanoparticles for bioanalysis by SERRS

D. Graham; Karen Faulds; David Thompson; Fiona McKenzie; Robert J. Stokes; Colette Dalton; Ross Stevenson; J. Alexander; Paul Garside; Emma McFarlane

doi:10.1042/BST0370697

Functionalized nanoparticles for bioanalysis by SERRS

D. Graham, Karen Faulds, David Thompson, Fiona McKenzie, Robert J. Stokes, Colette Dalton, Ross Stevenson, J. Alexander, Paul Garside, Emma McFarlane

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.

Original language	English
Pages (from-to)	697-701
Number of pages	5
Journal	Biochemical Society Transactions
Volume	37
DOIs	https://doi.org/10.1042/BST0370697
Publication status	Published - 22 Mar 2009

Keywords

bioanalysis
functionalized nanoparticle
immunoassay
metal surface
surface-enhanced resonance Raman scattering (SERRS)
surface plasmon resonance (SPR)

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10.1042/BST0370697

http://oasys2.confex.com/acs/237nm/techprogram/P1245712.HTM

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2 Finished

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture
Birch, D., Dawson, M., Faulds, K., Girkin, J., Graham, D., Martin, R., O'Donnell, K., Rolinski, O., Smith, W. & Wynne, K.
Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)
1/08/08 → 31/01/12
Project: Research
OPTICAL SPECTROSCOPIES FOR IMMUNO-BIOMEDICAL APPLICATIONS
Graham, D., Alexander, J., Birch, D., Faulds, K., Garside, P. & Smith, W.
EPSRC (Engineering and Physical Sciences Research Council)
1/08/06 → 31/01/12
Project: Research

Cite this

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title = "Functionalized nanoparticles for bioanalysis by SERRS",

abstract = "Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.",

keywords = "bioanalysis, functionalized nanoparticle, immunoassay, metal surface, surface-enhanced resonance Raman scattering (SERRS), surface plasmon resonance (SPR)",

author = "D. Graham and Karen Faulds and David Thompson and Fiona McKenzie and Stokes, {Robert J.} and Colette Dalton and Ross Stevenson and J. Alexander and Paul Garside and Emma McFarlane",

year = "2009",

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doi = "10.1042/BST0370697",

language = "English",

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pages = "697--701",

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T1 - Functionalized nanoparticles for bioanalysis by SERRS

AU - Graham, D.

AU - Faulds, Karen

AU - Thompson, David

AU - McKenzie, Fiona

AU - Stokes, Robert J.

AU - Dalton, Colette

AU - Stevenson, Ross

AU - Alexander, J.

AU - Garside, Paul

AU - McFarlane, Emma

PY - 2009/3/22

Y1 - 2009/3/22

N2 - Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.

AB - Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. In the present paper, we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach include DNA hybridization to switch on SERRS (surface-enhanced resonance Raman scattering) when a specific target sequence is present, the use of nanoparticles for in vivo SERRS imaging and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used in combination with SERRS.

KW - bioanalysis

KW - functionalized nanoparticle

KW - immunoassay

KW - metal surface

KW - surface-enhanced resonance Raman scattering (SERRS)

KW - surface plasmon resonance (SPR)

UR - http://oasys2.confex.com/acs/237nm/techprogram/P1245712.HTM

U2 - 10.1042/BST0370697

DO - 10.1042/BST0370697

M3 - Article

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SP - 697

EP - 701

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

ER -

Functionalized nanoparticles for bioanalysis by SERRS

Abstract

Keywords

Access to Document

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

OPTICAL SPECTROSCOPIES FOR IMMUNO-BIOMEDICAL APPLICATIONS

Cite this