Functionalised nanoparticles and SERS for bioanalysis

Duncan Graham, Karen Faulds, David Thompson, Fiona McKenzie, Colette Dalton, Anna Robson, Ross Stevenson

Research output: Contribution to journalBook/Film/Article review

Abstract

Metallic nanoparticles can be used as materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. Here we report how silver and gold nanoparticles can be functionalised with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach that will be discussed include DNA hybridisation to switch on surface enhanced resonance Raman scattering (SERRS) when a specific target sequence is present, recognition of specific proteins by aptamer functionalised nanoparticles by surface plasmon resonance and SERRS and use of nanoparticles functionalised with antibodies to provide a new type of immunoassay. In addition a new use of dip pen nanolithography in producing nanoarrays of biomolecules for detection by SERRS on a structured metal surface will be presented.
LanguageEnglish
Pages-
Number of pages1
JournalAbstracts of papers - American Chemical Society
Volume242
Publication statusPublished - 28 Aug 2011

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Nanoparticles
Raman scattering
Biomolecules
Nanolithography
Molecular recognition
Surface plasmon resonance
Silver
Antibodies
Gold
Labels
DNA
Metals
Fluorescence
Switches
Spectroscopy
Proteins
Molecules
Substrates

Keywords

  • SERS
  • bioanalysis
  • nanoparticles
  • TIC - Bionanotechnology

Cite this

Graham, D., Faulds, K., Thompson, D., McKenzie, F., Dalton, C., Robson, A., & Stevenson, R. (2011). Functionalised nanoparticles and SERS for bioanalysis. Abstracts of papers - American Chemical Society, 242, -.
Graham, Duncan ; Faulds, Karen ; Thompson, David ; McKenzie, Fiona ; Dalton, Colette ; Robson, Anna ; Stevenson, Ross. / Functionalised nanoparticles and SERS for bioanalysis. In: Abstracts of papers - American Chemical Society. 2011 ; Vol. 242. pp. -.
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Graham, D, Faulds, K, Thompson, D, McKenzie, F, Dalton, C, Robson, A & Stevenson, R 2011, 'Functionalised nanoparticles and SERS for bioanalysis' Abstracts of papers - American Chemical Society, vol. 242, pp. -.

Functionalised nanoparticles and SERS for bioanalysis. / Graham, Duncan; Faulds, Karen; Thompson, David; McKenzie, Fiona; Dalton, Colette; Robson, Anna; Stevenson, Ross.

In: Abstracts of papers - American Chemical Society, Vol. 242, 28.08.2011, p. -.

Research output: Contribution to journalBook/Film/Article review

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T1 - Functionalised nanoparticles and SERS for bioanalysis

AU - Graham, Duncan

AU - Faulds, Karen

AU - Thompson, David

AU - McKenzie, Fiona

AU - Dalton, Colette

AU - Robson, Anna

AU - Stevenson, Ross

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AB - Metallic nanoparticles can be used as materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. Here we report how silver and gold nanoparticles can be functionalised with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. Examples of this approach that will be discussed include DNA hybridisation to switch on surface enhanced resonance Raman scattering (SERRS) when a specific target sequence is present, recognition of specific proteins by aptamer functionalised nanoparticles by surface plasmon resonance and SERRS and use of nanoparticles functionalised with antibodies to provide a new type of immunoassay. In addition a new use of dip pen nanolithography in producing nanoarrays of biomolecules for detection by SERRS on a structured metal surface will be presented.

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