FTIR-based spectroscopic analysis in the identification of clinically aggressive prostate cancer

M. J. Baker, E. Gazi, M. D. Brown, J. H. Shanks, P. Gardner, N. W. Clarke

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Fourier transform infrared (FTIR) spectroscopy is a vibrational spectroscopic technique that uses infrared radiation to vibrate molecular bonds within the sample that absorbs it. As different samples contain different molecular bonds or different configurations of molecular bonds, FTIR allows us to obtain chemical information on molecules within the sample. Fourier transform infrared microspectroscopy in conjunction with a principal component- discriminant function analysis (PC-DFA) algorithm was applied to the grading of prostate cancer (CaP) tissue specimens. The PC-DFA algorithm is used alongside the established diagnostic measures of Gleason grading and the tumour/node/metastasis system. Principal component-discriminant function analysis improved the sensitivity and specificity of a three-band Gleason score criterion diagnosis previously reported by attaining an overall sensitivity of 92.3% and specificity of 99.4%. For the first time, we present the use of a two-band criterion showing an association of FTIR-based spectral characteristics with clinically aggressive behaviour in CaP manifest as local and/or distal spread. This paper shows the potential for the use of spectroscopic analysis for the evaluation of the biopotential of CaP in an accurate and reproducible manner.

LanguageEnglish
Pages1859-1866
Number of pages8
JournalBritish Journal of Cancer
Volume99
Issue number11
Early online date4 Nov 2008
DOIs
Publication statusPublished - 2 Dec 2008

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Discriminant Analysis
Fourier Analysis
Prostatic Neoplasms
Neoplasm Grading
Molecular Conformation
Sensitivity and Specificity
Fourier Transform Infrared Spectroscopy
Radiation
Neoplasm Metastasis
Neoplasms

Keywords

  • FTIR microspectroscopy
  • Gleason grade
  • infrared spectroscopy
  • molecular diagnosis
  • PC-DFA
  • prostate cancer

Cite this

Baker, M. J. ; Gazi, E. ; Brown, M. D. ; Shanks, J. H. ; Gardner, P. ; Clarke, N. W. / FTIR-based spectroscopic analysis in the identification of clinically aggressive prostate cancer. In: British Journal of Cancer. 2008 ; Vol. 99, No. 11. pp. 1859-1866.
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FTIR-based spectroscopic analysis in the identification of clinically aggressive prostate cancer. / Baker, M. J.; Gazi, E.; Brown, M. D.; Shanks, J. H.; Gardner, P.; Clarke, N. W.

In: British Journal of Cancer, Vol. 99, No. 11, 02.12.2008, p. 1859-1866.

Research output: Contribution to journalArticle

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