From single-molecule spectroscopy to super-resolution imaging of the neuron

a review

Romain F Laine, Gabriele S Kaminski Schierle, Sebastian van de Linde, Clemens F Kaminski

Research output: Contribution to journalArticle

11 Citations (Scopus)
20 Downloads (Pure)

Abstract

For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.
Original languageEnglish
Article number022004
Number of pages12
JournalMethods and Applications in Fluorescence
Volume4
Issue number2
DOIs
Publication statusPublished - 24 Jun 2016

Fingerprint

neurons
Neurons
Spectroscopy
Imaging techniques
Molecules
Neurosciences
spectroscopy
molecules
Microscopy
neurology
Computational methods
acceleration (physics)
Research
Microscopic examination
Diffraction
microscopy
Single Molecule Imaging
cells
diffraction

Keywords

  • single-molecule spectroscopy
  • super-resolution imaging
  • neuroscience research

Cite this

Laine, Romain F ; Schierle, Gabriele S Kaminski ; van de Linde, Sebastian ; Kaminski, Clemens F. / From single-molecule spectroscopy to super-resolution imaging of the neuron : a review. In: Methods and Applications in Fluorescence . 2016 ; Vol. 4, No. 2.
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From single-molecule spectroscopy to super-resolution imaging of the neuron : a review. / Laine, Romain F; Schierle, Gabriele S Kaminski; van de Linde, Sebastian; Kaminski, Clemens F.

In: Methods and Applications in Fluorescence , Vol. 4, No. 2, 022004, 24.06.2016.

Research output: Contribution to journalArticle

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