From emulsion to single-phase microfluidics: an integrated approach to culture and perfusion of multicellular spheroids

Kay S McMillan, Marie Boyd, Michele Zagnoni

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

This study presents a novel microfluidic approach for developing large scale screening assays of anticancer compounds on 3D multicellular spheroids. We have developed a microfluidic device with associated protocols that combine the high-throughput characteristics of droplet microfluidics for spheroid formation and aggregation with those of single-phase microfluidics for substance exchange, long term culture and drug perfusion.
LanguageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of PublicationSan Diego, CA
Pages399-400
Number of pages2
Publication statusPublished - 9 Oct 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Center Dublin, Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016
http://www.microtas2016.org/

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated titleMicroTas 2016
CountryIreland
CityDublin
Period9/10/1613/10/16
Internet address

Fingerprint

Cellular Spheroids
Microfluidics
Emulsions
Perfusion
Lab-On-A-Chip Devices
Pharmaceutical Preparations

Keywords

  • droplet microfluidics
  • spheroids
  • cancer
  • 3D cell culture

Cite this

McMillan, K. S., Boyd, M., & Zagnoni, M. (2016). From emulsion to single-phase microfluidics: an integrated approach to culture and perfusion of multicellular spheroids. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) (pp. 399-400). San Diego, CA.
McMillan, Kay S ; Boyd, Marie ; Zagnoni, Michele. / From emulsion to single-phase microfluidics : an integrated approach to culture and perfusion of multicellular spheroids. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, 2016. pp. 399-400
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McMillan, KS, Boyd, M & Zagnoni, M 2016, From emulsion to single-phase microfluidics: an integrated approach to culture and perfusion of multicellular spheroids. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, pp. 399-400, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Dublin, Ireland, 9/10/16.

From emulsion to single-phase microfluidics : an integrated approach to culture and perfusion of multicellular spheroids. / McMillan, Kay S; Boyd, Marie; Zagnoni, Michele.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, 2016. p. 399-400.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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AU - Zagnoni, Michele

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KW - droplet microfluidics

KW - spheroids

KW - cancer

KW - 3D cell culture

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McMillan KS, Boyd M, Zagnoni M. From emulsion to single-phase microfluidics: an integrated approach to culture and perfusion of multicellular spheroids. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA. 2016. p. 399-400