FRET measurements on fuzzy fluorescent nanostructures

V. Caorsi, E. Ronzitti, G. Vicidomini, S. Krol, G. McConnell, A. Diaspro

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In the last decade, fluorescence resonance energy transfer (FRET) has become a useful technique for studying intermolecular interactions applied to the analysis of biological systems. Although FRET measurements may be very helpful in the comprehension of different cellular processes, it can be difficult to obtain quantitative results, hence the necessity of studying FRET on controllable systems. Here, a fuzzy nanostructured system called a nanocapsule is presented as a nanometric-device allowing distance modulation, thus preserving photophysical properties of fluorescent dyes and exhibiting good potential features for improving quantitative FRET analysis. We evaluated the behavior of such a sample using four FRET methods (three of them based on steady-state fluorescence and one using lifetime measurements). Within some limitations that can be overcome, these nanodevices have the potential to serve as a benchmark system for characterizing new FRET couples and to develop quantitative approaches for FRET analysis.

LanguageEnglish
Pages452-458
Number of pages7
JournalMicroscopy Research and Technique
Volume70
Issue number5
DOIs
Publication statusPublished - May 2007

Fingerprint

Fluorescence Resonance Energy Transfer
Nanostructures
resonance fluorescence
energy transfer
Nanocapsules
fuzzy systems
Benchmarking
Biological systems
Systems Analysis
Fluorescent Dyes
preserving
Dyes
Fluorescence
dyes
Modulation
modulation
Equipment and Supplies
life (durability)
fluorescence

Keywords

  • FRET
  • FLIM
  • acceptor photobleaching
  • spectral bleed through
  • supercontinuum
  • nanocapsules
  • resonance energy-transfer
  • transfer microscopy
  • photon
  • laser
  • cells

Cite this

Caorsi, V., Ronzitti, E., Vicidomini, G., Krol, S., McConnell, G., & Diaspro, A. (2007). FRET measurements on fuzzy fluorescent nanostructures. Microscopy Research and Technique, 70(5), 452-458. https://doi.org/10.1002/jemt.20444
Caorsi, V. ; Ronzitti, E. ; Vicidomini, G. ; Krol, S. ; McConnell, G. ; Diaspro, A. / FRET measurements on fuzzy fluorescent nanostructures. In: Microscopy Research and Technique. 2007 ; Vol. 70, No. 5. pp. 452-458.
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Caorsi, V, Ronzitti, E, Vicidomini, G, Krol, S, McConnell, G & Diaspro, A 2007, 'FRET measurements on fuzzy fluorescent nanostructures' Microscopy Research and Technique, vol. 70, no. 5, pp. 452-458. https://doi.org/10.1002/jemt.20444

FRET measurements on fuzzy fluorescent nanostructures. / Caorsi, V.; Ronzitti, E.; Vicidomini, G.; Krol, S.; McConnell, G.; Diaspro, A.

In: Microscopy Research and Technique, Vol. 70, No. 5, 05.2007, p. 452-458.

Research output: Contribution to journalArticle

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AU - Caorsi, V.

AU - Ronzitti, E.

AU - Vicidomini, G.

AU - Krol, S.

AU - McConnell, G.

AU - Diaspro, A.

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KW - laser

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