A new integration based fluorescence lifetime imaging microscopy (FLIM) called IEM has been proposed to implement lifetime calculations . A real-time hardware implementation of this IEM FLIM algorithm suitable for a single photon avalanche diode (SPAD) array in 0.13µm CMOS technology is now implemented on FPGA. A widefield microscope was adapted to accommodate the array and test it on biological applications. Video-rate fluorescence lifetime imaging has been achieved, by performing parallel 32×32 lifetime calculations, realizing the first, compact, and low-cost FLIM camera.
|Conference|| IEEE International Symposium on Circuits and Systems, 2009|
|Abbreviated title||ISCAS 2009|
|Period||24/05/09 → 27/05/09|
- field programmable gate arrays
- CMOS technology
- adaptive arrays