Fourier-transform infrared spectroscopy of biofluids: a practical approach

Ashton G. Theakstone, Christopher Rinaldi, Holly J. Butler, James M. Cameron, Lily Rose Confield, Samantha H. Rutherford, Alexandra Sala, Sayali Sangamnerkar, Matthew J. Baker

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Biofluid spectroscopy is an emerging technology in the field of clinical investigation, providing a simple way to extract diagnostic and observational information from easy to acquire samples. Infrared spectroscopy is well suited to analyse a large range of biofluid samples, including blood and its derivatives, due to flexible sampling modes and high sensitivity to subtle biological changes. As the technology advances towards the clinic, factors influencing successful clinical translation are becoming apparent. Here, we provide a tutorial for effective biofluid spectroscopy study design, discussing sample and instrument parameters, as well as clinical considerations. The aim is to present the current understanding of clinical translation in the field of biofluid spectroscopy, and to facilitate other clinical applications to advance to the clinic.
Original languageEnglish
Article numbere202000025
Number of pages20
JournalTranslational Biophotonics
Issue number2
Early online date16 Jan 2021
Publication statusPublished - 31 May 2021


  • biofluids
  • chemometrics
  • clinical translation
  • infrared
  • vibrational spectroscopy


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