Formulation of Nonionic Surfactant Vesicles (NISV) prepared by microfluidics for therapeutic delivery of siRNA into cancer cells

Mohammad A. Obeid; Ashref Elburi; Louise C. Young; Alexander B. Mullen; Rothwelle J. Tate; Valerie A. Ferro

doi:10.1021/acs.molpharmaceut.7b00352

Formulation of Nonionic Surfactant Vesicles (NISV) prepared by microfluidics for therapeutic delivery of siRNA into cancer cells

Mohammad A. Obeid^*, Ashref Elburi, Louise C. Young, Alexander B. Mullen, Rothwelle J. Tate, Valerie A. Ferro

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

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Abstract

Small interfering RNAs (siRNA) have a broad potential as therapeutic agents to reversibly silence any target gene of interest. The clinical application of siRNA requires the use of safe and effective delivery systems. In this study, we investigated the use of nonionic surfactant vesicles (NISV) for the delivery of siRNA. Different types of NISV formulations were synthesized by microfluidic mixing and then evaluated for their physiochemical properties and cytotoxicity. The ability of the NISV to carry and transfect siRNA targeting green fluorescent protein (GFP) into A549 that stably express GFP (copGFP-A549) was evaluated. Flow cytometry and Western blotting were used to study the GFP expression knockdown, and significant knockdown was observed as a result of siRNA delivery to the cells by NISV. This occurred in particular when using Tween 85, which was able to achieve more than 70% GFP knockdown. NISV were thus demonstrated to provide a promising and effective platform for therapeutic delivery of siRNA.

Original language	English
Pages (from-to)	2450-2458
Number of pages	9
Journal	Molecular Pharmaceutics
Volume	14
Issue number	7
Early online date	1 Jun 2017
DOIs	https://doi.org/10.1021/acs.molpharmaceut.7b00352
Publication status	Published - 3 Jul 2017

Keywords

drug delivery
microfluidics
nonionic surfactant vesicles
RNA interference

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10.1021/acs.molpharmaceut.7b00352

Obeid-etal-MP-2017-Formulation-of-Nonionic-Surfactant-Vesicles-NISV-prepared-by-microfluidicsAccepted author manuscript, 1.16 MB

Valerie Ferro
- v.a.ferrostrath.acuk
- Strathclyde Institute Of Pharmacy And Biomedical Sciences - Senior Lecturer
Person: Academic

35 Citations
1 Article

Comparison of the physical characteristics of monodisperse non-ionic surfactant vesicles (NISV) prepared using different manufacturing methods
Obeid, M. A., Gebril, A. M., Tate, R. J., Mullen, A. B. & Ferro, V. A., 3 Feb 2017, (E-pub ahead of print) In: International Journal of Pharmaceutics. p. 1-23 23 p.
Research output: Contribution to journal › Article › peer-review

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28 Citations (Scopus)

81 Downloads (Pure)

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title = "Formulation of Nonionic Surfactant Vesicles (NISV) prepared by microfluidics for therapeutic delivery of siRNA into cancer cells",

abstract = "Small interfering RNAs (siRNA) have a broad potential as therapeutic agents to reversibly silence any target gene of interest. The clinical application of siRNA requires the use of safe and effective delivery systems. In this study, we investigated the use of nonionic surfactant vesicles (NISV) for the delivery of siRNA. Different types of NISV formulations were synthesized by microfluidic mixing and then evaluated for their physiochemical properties and cytotoxicity. The ability of the NISV to carry and transfect siRNA targeting green fluorescent protein (GFP) into A549 that stably express GFP (copGFP-A549) was evaluated. Flow cytometry and Western blotting were used to study the GFP expression knockdown, and significant knockdown was observed as a result of siRNA delivery to the cells by NISV. This occurred in particular when using Tween 85, which was able to achieve more than 70% GFP knockdown. NISV were thus demonstrated to provide a promising and effective platform for therapeutic delivery of siRNA.",

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AU - Obeid, Mohammad A.

AU - Elburi, Ashref

AU - Young, Louise C.

AU - Mullen, Alexander B.

AU - Tate, Rothwelle J.

AU - Ferro, Valerie A.

PY - 2017/7/3

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AB - Small interfering RNAs (siRNA) have a broad potential as therapeutic agents to reversibly silence any target gene of interest. The clinical application of siRNA requires the use of safe and effective delivery systems. In this study, we investigated the use of nonionic surfactant vesicles (NISV) for the delivery of siRNA. Different types of NISV formulations were synthesized by microfluidic mixing and then evaluated for their physiochemical properties and cytotoxicity. The ability of the NISV to carry and transfect siRNA targeting green fluorescent protein (GFP) into A549 that stably express GFP (copGFP-A549) was evaluated. Flow cytometry and Western blotting were used to study the GFP expression knockdown, and significant knockdown was observed as a result of siRNA delivery to the cells by NISV. This occurred in particular when using Tween 85, which was able to achieve more than 70% GFP knockdown. NISV were thus demonstrated to provide a promising and effective platform for therapeutic delivery of siRNA.

KW - drug delivery

KW - microfluidics

KW - nonionic surfactant vesicles

KW - RNA interference

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Formulation of Nonionic Surfactant Vesicles (NISV) prepared by microfluidics for therapeutic delivery of siRNA into cancer cells

Abstract

Keywords

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Valerie Ferro

Research output

Comparison of the physical characteristics of monodisperse non-ionic surfactant vesicles (NISV) prepared using different manufacturing methods

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