Formation of artificial lipid bilayers using droplet dielectrophoresis

Sara Aghdaei; M.E. Sandison; Michele Zagnoni; Nicolas G. Green; Hywel Morgan

doi:10.1039/b807374k

Formation of artificial lipid bilayers using droplet dielectrophoresis

Sara Aghdaei, M.E. Sandison, Michele Zagnoni, Nicolas G. Green, Hywel Morgan

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Abstract

We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

Original language	English
Pages (from-to)	1617–1620
Number of pages	5
Journal	Lab on a Chip
Volume	8
Issue number	10
Early online date	13 Aug 2008
DOIs	https://doi.org/10.1039/b807374k
Publication status	Published - Oct 2008

Keywords

droplet movement
artificial lipid bilayers
lipid bilayers
droplets

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10.1039/b807374k

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abstract = "We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.",

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author = "Sara Aghdaei and M.E. Sandison and Michele Zagnoni and Green, \{Nicolas G.\} and Hywel Morgan",

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AU - Aghdaei, Sara

AU - Sandison, M.E.

AU - Zagnoni, Michele

AU - Green, Nicolas G.

AU - Morgan, Hywel

PY - 2008/10

Y1 - 2008/10

N2 - We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

AB - We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

KW - droplet movement

KW - artificial lipid bilayers

KW - lipid bilayers

KW - droplets

U2 - 10.1039/b807374k

DO - 10.1039/b807374k

M3 - Article

SN - 1473-0197

VL - 8

SP - 1617

EP - 1620

JO - Lab on a Chip

JF - Lab on a Chip

IS - 10

ER -

Formation of artificial lipid bilayers using droplet dielectrophoresis

Abstract

Keywords

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