Formation of artificial lipid bilayers using droplet dielectrophoresis

Sara Aghdaei; M.E. Sandison; Michele Zagnoni; Nicolas G. Green; Hywel Morgan

doi:10.1039/b807374k

Formation of artificial lipid bilayers using droplet dielectrophoresis

Sara Aghdaei, M.E. Sandison, Michele Zagnoni, Nicolas G. Green, Hywel Morgan

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

Original language	English
Pages (from-to)	1617–1620
Number of pages	5
Journal	Lab on a Chip
Volume	8
Issue number	10
Early online date	13 Aug 2008
DOIs	https://doi.org/10.1039/b807374k
Publication status	Published - Oct 2008

Keywords

droplet movement
artificial lipid bilayers
lipid bilayers
droplets

Access to Document

10.1039/b807374k

Cite this

@article{c8eefa9e34fe45af9e45038553212d19,

title = "Formation of artificial lipid bilayers using droplet dielectrophoresis",

abstract = "We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.",

keywords = "droplet movement , artificial lipid bilayers, lipid bilayers , droplets",

author = "Sara Aghdaei and M.E. Sandison and Michele Zagnoni and Green, {Nicolas G.} and Hywel Morgan",

year = "2008",

month = oct,

doi = "10.1039/b807374k",

language = "English",

volume = "8",

pages = "1617–1620 ",

journal = "Lab on a Chip",

issn = "1473-0197",

publisher = "Royal Society of Chemistry",

number = "10",

}

TY - JOUR

T1 - Formation of artificial lipid bilayers using droplet dielectrophoresis

AU - Aghdaei, Sara

AU - Sandison, M.E.

AU - Zagnoni, Michele

AU - Green, Nicolas G.

AU - Morgan, Hywel

PY - 2008/10

Y1 - 2008/10

N2 - We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

AB - We describe the formation of artificial bilayer lipid membranes (BLMs) by the controlled, electrical manipulation of aqueous droplets immersed in a lipid–alkane solution. Droplet movement was generated using dielectrophoresis on planar microelectrodes covered in a thin insulator. Droplets, surrounded by lipid monolayers, were brought into contact and spontaneously formed a BLM. The method produced BLMs suitable for single-channel recording of membrane protein activity and the technique can be extended to create programmable BLM arrays and networks.

KW - droplet movement

KW - artificial lipid bilayers

KW - lipid bilayers

KW - droplets

U2 - 10.1039/b807374k

DO - 10.1039/b807374k

M3 - Article

SN - 1473-0197

VL - 8

SP - 1617

EP - 1620

JO - Lab on a Chip

JF - Lab on a Chip

IS - 10

ER -

Formation of artificial lipid bilayers using droplet dielectrophoresis

Abstract

Keywords

Access to Document

Fingerprint

Cite this