Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system

Nikolay Dimov, Elisabeth Kastner, Maryam Tabassum Hussain, Yvonne Perrie, Nicolas Szita

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body and they are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are much needed. Here we present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300 nm) varying in lipid composition, size and charge. This system offers effective and rapid nanomedicine purification with high lipid recovery (>98%) combined with effective removal of non-entrapped drug (propofol >95% reduction of non-entrapped drug present) or protein (ovalbumin >90% reduction of OVA present) and organic solvent (ethanol >95% reduction) in less than 4 minutes. Within this manuscript, we outline a new set-up that offer the key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.
LanguageEnglish
Article number12045
Number of pages19
JournalScientific Reports
Volume7
Issue number1
Early online date21 Sep 2017
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Nanomedicine
Liposomes
Pharmaceutical Preparations
Lipids
Ovalbumin
Lipid Bilayers
Propofol
Nanoparticles
Ethanol
Costs and Cost Analysis
Membranes
Proteins

Keywords

  • liposomes
  • microfluidics
  • continuous-flow
  • drug delivery

Cite this

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abstract = "Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body and they are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are much needed. Here we present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300 nm) varying in lipid composition, size and charge. This system offers effective and rapid nanomedicine purification with high lipid recovery (>98{\%}) combined with effective removal of non-entrapped drug (propofol >95{\%} reduction of non-entrapped drug present) or protein (ovalbumin >90{\%} reduction of OVA present) and organic solvent (ethanol >95{\%} reduction) in less than 4 minutes. Within this manuscript, we outline a new set-up that offer the key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.",
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Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system. / Dimov, Nikolay; Kastner, Elisabeth; Hussain, Maryam Tabassum; Perrie, Yvonne; Szita, Nicolas.

In: Scientific Reports, Vol. 7, No. 1, 12045, 01.12.2017.

Research output: Contribution to journalArticle

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