Forces acting on biological cells in external electrical fields

Research output: Contribution to conferencePaper

6 Citations (Scopus)

Abstract

Biological cells stressed by external electric fields undergo mechanical deformation and remodeling caused by electro-mechanical Maxwell stresses. These stresses appear at the internal and external membrane interfaces due to differences in dielectric properties of the biological membrane and the surrounding and internal fluids. Electro-mechanical loading of the cells results in the reversible or irreversible development of pores in the membrane. This could cause disruption in normal functioning of the cell and even leads to cell death. Such a phenomenon has several practical applications including pulsed electric field (PEF) processing of fluids. In the PEF process liquids and pumpable products are subjected to short high electric field pulses that lead to non-thermal inactivation of bacteria. In the present paper the dynamics of electromechanical loading of spherical cells in external pulsed electric fields has been considered. The cell was modeled as a simple insulating dielectric shell containing a conducting fluid. Cells were suspended in a second conducting fluid. For a single spherical cell electrical fields and electro-mechanical forces have been calculated both analytically and using finite-element software. The transient electrical fields induced in a cell membrane subjected to a high- field step pulse are increased due to Maxwell-Wagner polarization effects at the membrane/conductive fluid interfaces. The electro-mechanical forces acting on the cell also grow during this transient polarization process, the duration depending on the conductivities and permittivities of the inter-cellular and extra-cellular fluids. As a result the electro-mechanical stresses in the membrane reach a maximal value up to three orders of magnitude higher than the initial stress. This maximum value occurred in a time comparable to the Maxwell-Wagner relaxation time of the conducting fluid. In the case of two spherical cells located near to each other the finite difference software was used to calculate the induced stresses. It was shown that the field and electro-mechanical stresses in the two-cell model were practically identical to the single sphere model.

Conference

ConferenceAnnual Conference on Electrical Insulation and Dielectric Phenomena
CountryUnited States
CityKansas City
Period15/10/0618/10/06

Fingerprint

cells
conducting fluids
membranes
electric fields
fluids
computer programs
polarization
pulses
death
deactivation
bacteria
dielectric properties
relaxation time
permittivity
porosity
conductivity
causes
products
liquids

Keywords

  • membranes
  • pulses
  • biological cells
  • external electrical fields

Cite this

Timoshkin, I., MacGregor, S. J., Fouracre, R. A., Given, M. J., & Anderson, J. G. (2006). Forces acting on biological cells in external electrical fields. 676-679. Paper presented at Annual Conference on Electrical Insulation and Dielectric Phenomena , Kansas City, United States. https://doi.org/10.1109/CEIDP.2006.312022
Timoshkin, I. ; MacGregor, S.J. ; Fouracre, R.A. ; Given, M.J. ; Anderson, J.G. / Forces acting on biological cells in external electrical fields. Paper presented at Annual Conference on Electrical Insulation and Dielectric Phenomena , Kansas City, United States.3 p.
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abstract = "Biological cells stressed by external electric fields undergo mechanical deformation and remodeling caused by electro-mechanical Maxwell stresses. These stresses appear at the internal and external membrane interfaces due to differences in dielectric properties of the biological membrane and the surrounding and internal fluids. Electro-mechanical loading of the cells results in the reversible or irreversible development of pores in the membrane. This could cause disruption in normal functioning of the cell and even leads to cell death. Such a phenomenon has several practical applications including pulsed electric field (PEF) processing of fluids. In the PEF process liquids and pumpable products are subjected to short high electric field pulses that lead to non-thermal inactivation of bacteria. In the present paper the dynamics of electromechanical loading of spherical cells in external pulsed electric fields has been considered. The cell was modeled as a simple insulating dielectric shell containing a conducting fluid. Cells were suspended in a second conducting fluid. For a single spherical cell electrical fields and electro-mechanical forces have been calculated both analytically and using finite-element software. The transient electrical fields induced in a cell membrane subjected to a high- field step pulse are increased due to Maxwell-Wagner polarization effects at the membrane/conductive fluid interfaces. The electro-mechanical forces acting on the cell also grow during this transient polarization process, the duration depending on the conductivities and permittivities of the inter-cellular and extra-cellular fluids. As a result the electro-mechanical stresses in the membrane reach a maximal value up to three orders of magnitude higher than the initial stress. This maximum value occurred in a time comparable to the Maxwell-Wagner relaxation time of the conducting fluid. In the case of two spherical cells located near to each other the finite difference software was used to calculate the induced stresses. It was shown that the field and electro-mechanical stresses in the two-cell model were practically identical to the single sphere model.",
keywords = "membranes, pulses, biological cells, external electrical fields",
author = "I. Timoshkin and S.J. MacGregor and R.A. Fouracre and M.J. Given and J.G. Anderson",
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note = "Annual Conference on Electrical Insulation and Dielectric Phenomena ; Conference date: 15-10-2006 Through 18-10-2006",

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Timoshkin, I, MacGregor, SJ, Fouracre, RA, Given, MJ & Anderson, JG 2006, 'Forces acting on biological cells in external electrical fields' Paper presented at Annual Conference on Electrical Insulation and Dielectric Phenomena , Kansas City, United States, 15/10/06 - 18/10/06, pp. 676-679. https://doi.org/10.1109/CEIDP.2006.312022

Forces acting on biological cells in external electrical fields. / Timoshkin, I.; MacGregor, S.J.; Fouracre, R.A.; Given, M.J.; Anderson, J.G.

2006. 676-679 Paper presented at Annual Conference on Electrical Insulation and Dielectric Phenomena , Kansas City, United States.

Research output: Contribution to conferencePaper

TY - CONF

T1 - Forces acting on biological cells in external electrical fields

AU - Timoshkin, I.

AU - MacGregor, S.J.

AU - Fouracre, R.A.

AU - Given, M.J.

AU - Anderson, J.G.

PY - 2006/10

Y1 - 2006/10

N2 - Biological cells stressed by external electric fields undergo mechanical deformation and remodeling caused by electro-mechanical Maxwell stresses. These stresses appear at the internal and external membrane interfaces due to differences in dielectric properties of the biological membrane and the surrounding and internal fluids. Electro-mechanical loading of the cells results in the reversible or irreversible development of pores in the membrane. This could cause disruption in normal functioning of the cell and even leads to cell death. Such a phenomenon has several practical applications including pulsed electric field (PEF) processing of fluids. In the PEF process liquids and pumpable products are subjected to short high electric field pulses that lead to non-thermal inactivation of bacteria. In the present paper the dynamics of electromechanical loading of spherical cells in external pulsed electric fields has been considered. The cell was modeled as a simple insulating dielectric shell containing a conducting fluid. Cells were suspended in a second conducting fluid. For a single spherical cell electrical fields and electro-mechanical forces have been calculated both analytically and using finite-element software. The transient electrical fields induced in a cell membrane subjected to a high- field step pulse are increased due to Maxwell-Wagner polarization effects at the membrane/conductive fluid interfaces. The electro-mechanical forces acting on the cell also grow during this transient polarization process, the duration depending on the conductivities and permittivities of the inter-cellular and extra-cellular fluids. As a result the electro-mechanical stresses in the membrane reach a maximal value up to three orders of magnitude higher than the initial stress. This maximum value occurred in a time comparable to the Maxwell-Wagner relaxation time of the conducting fluid. In the case of two spherical cells located near to each other the finite difference software was used to calculate the induced stresses. It was shown that the field and electro-mechanical stresses in the two-cell model were practically identical to the single sphere model.

AB - Biological cells stressed by external electric fields undergo mechanical deformation and remodeling caused by electro-mechanical Maxwell stresses. These stresses appear at the internal and external membrane interfaces due to differences in dielectric properties of the biological membrane and the surrounding and internal fluids. Electro-mechanical loading of the cells results in the reversible or irreversible development of pores in the membrane. This could cause disruption in normal functioning of the cell and even leads to cell death. Such a phenomenon has several practical applications including pulsed electric field (PEF) processing of fluids. In the PEF process liquids and pumpable products are subjected to short high electric field pulses that lead to non-thermal inactivation of bacteria. In the present paper the dynamics of electromechanical loading of spherical cells in external pulsed electric fields has been considered. The cell was modeled as a simple insulating dielectric shell containing a conducting fluid. Cells were suspended in a second conducting fluid. For a single spherical cell electrical fields and electro-mechanical forces have been calculated both analytically and using finite-element software. The transient electrical fields induced in a cell membrane subjected to a high- field step pulse are increased due to Maxwell-Wagner polarization effects at the membrane/conductive fluid interfaces. The electro-mechanical forces acting on the cell also grow during this transient polarization process, the duration depending on the conductivities and permittivities of the inter-cellular and extra-cellular fluids. As a result the electro-mechanical stresses in the membrane reach a maximal value up to three orders of magnitude higher than the initial stress. This maximum value occurred in a time comparable to the Maxwell-Wagner relaxation time of the conducting fluid. In the case of two spherical cells located near to each other the finite difference software was used to calculate the induced stresses. It was shown that the field and electro-mechanical stresses in the two-cell model were practically identical to the single sphere model.

KW - membranes

KW - pulses

KW - biological cells

KW - external electrical fields

UR - http://dx.doi.org/10.1109/CEIDP.2006.312022

U2 - 10.1109/CEIDP.2006.312022

DO - 10.1109/CEIDP.2006.312022

M3 - Paper

SP - 676

EP - 679

ER -

Timoshkin I, MacGregor SJ, Fouracre RA, Given MJ, Anderson JG. Forces acting on biological cells in external electrical fields. 2006. Paper presented at Annual Conference on Electrical Insulation and Dielectric Phenomena , Kansas City, United States. https://doi.org/10.1109/CEIDP.2006.312022