Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

K. Kosmidis; Christopher D. Russell; George Dobre; Adrian Gh. Podoleanu; R.A. Black

doi:10.1089/ten.2007.1501

Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

K. Kosmidis, Christopher D. Russell, George Dobre, Adrian Gh. Podoleanu, R.A. Black

Research output: Contribution to journal › Article › peer-review

Abstract

The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

Original language	English
Pages (from-to)	60790Q-1
Journal	Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE)
Volume	6079
DOIs	https://doi.org/10.1089/ten.2007.1501
Publication status	Published - 2007

Keywords

optical coherence tomography
collagen gels
cell distribution
tissue engineering

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10.1089/ten.2007.1501

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@article{1f6fbdb7ce4d4a39af126e59ab10804c,

title = "Flying spot en face OCT for monitoring cell distribution in collagen-based constructs",

abstract = "The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.",

keywords = "optical coherence tomography, collagen gels, cell distribution, tissue engineering",

author = "K. Kosmidis and Russell, \{Christopher D.\} and George Dobre and Podoleanu, \{Adrian Gh.\} and R.A. Black",

note = "Biomedical Optics Conference, San Jose, 21-26 Jan. 2006 Progress in Biomedical Optics and Imaging, Vol. 7, No. 2, 2006",

year = "2007",

doi = "10.1089/ten.2007.1501",

language = "English",

volume = "6079",

pages = "60790Q--1",

journal = "Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE)",

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TY - JOUR

T1 - Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

AU - Kosmidis, K.

AU - Russell, Christopher D.

AU - Dobre, George

AU - Podoleanu, Adrian Gh.

AU - Black, R.A.

N1 - Biomedical Optics Conference, San Jose, 21-26 Jan. 2006 Progress in Biomedical Optics and Imaging, Vol. 7, No. 2, 2006

PY - 2007

Y1 - 2007

N2 - The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

AB - The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

KW - optical coherence tomography

KW - collagen gels

KW - cell distribution

KW - tissue engineering

UR - http://dx.doi.org/10.1089/ten.2007.1501

U2 - 10.1089/ten.2007.1501

DO - 10.1089/ten.2007.1501

M3 - Article

SN - 1605-7422

VL - 6079

SP - 60790Q-1

JO - Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE)

JF - Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE)

ER -

Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

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Keywords

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