The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.
|Journal||Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE)|
|Publication status||Published - 2007|
- optical coherence tomography
- collagen gels
- cell distribution
- tissue engineering
Kosmidis, K., Russell, C. D., Dobre, G., Podoleanu, A. G., & Black, R. A. (2007). Flying spot en face OCT for monitoring cell distribution in collagen-based constructs. Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE), 6079, 60790Q-1. https://doi.org/10.1089/ten.2007.1501