Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

K. Kosmidis, Christopher D. Russell, George Dobre, Adrian Gh. Podoleanu, R.A. Black

    Research output: Contribution to journalArticle

    Abstract

    The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.
    Original languageEnglish
    Pages (from-to)60790Q-1
    JournalMultiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE)
    Volume6079
    DOIs
    Publication statusPublished - 2007

    Keywords

    • optical coherence tomography
    • collagen gels
    • cell distribution
    • tissue engineering

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