Flying spot en face OCT for monitoring cell distribution in collagen-based constructs

K. Kosmidis, Christopher D. Russell, George Dobre, Adrian Gh. Podoleanu, R.A. Black

    Research output: Contribution to journalArticle

    Abstract

    The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.
    LanguageEnglish
    Pages60790Q-1
    JournalMultiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE)
    Volume6079
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Optical Coherence Tomography
    Collagen
    Optical tomography
    collagens
    tomography
    flight
    Monitoring
    Fibroblasts
    Cell
    fibroblasts
    cells
    Cells
    tendons
    Tendons
    Collagen Type I
    rats
    contraction
    Tail
    Rats
    Contraction

    Keywords

    • optical coherence tomography
    • collagen gels
    • cell distribution
    • tissue engineering

    Cite this

    Kosmidis, K. ; Russell, Christopher D. ; Dobre, George ; Podoleanu, Adrian Gh. ; Black, R.A. / Flying spot en face OCT for monitoring cell distribution in collagen-based constructs. In: Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE). 2007 ; Vol. 6079. pp. 60790Q-1.
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    abstract = "The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.",
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    author = "K. Kosmidis and Russell, {Christopher D.} and George Dobre and Podoleanu, {Adrian Gh.} and R.A. Black",
    note = "Biomedical Optics Conference, San Jose, 21-26 Jan. 2006 Progress in Biomedical Optics and Imaging, Vol. 7, No. 2, 2006",
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    Flying spot en face OCT for monitoring cell distribution in collagen-based constructs. / Kosmidis, K.; Russell, Christopher D.; Dobre, George; Podoleanu, Adrian Gh.; Black, R.A.

    In: Multiphoton Microscopy in the Biomedical Sciences IV (Proceedings of SPIE), Vol. 6079, 2007, p. 60790Q-1.

    Research output: Contribution to journalArticle

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    AU - Russell, Christopher D.

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    AU - Black, R.A.

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    KW - cell distribution

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