Fluorous-directed assembly of DNA origami nanostructures

Jiajia Zou, Ashley C. Stammers, Andrea Taladriz-Sender, Jamie M. Withers, Iain Christie, Marina Santana Vega, Badri L. Aekbote, William J. Peveler, David A. Rusling, Glenn A. Burley, Alasdair W. Clark

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
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An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.
Original languageEnglish
Pages (from-to)752–759
Number of pages8
JournalACS Nano
Issue number1
Early online date20 Dec 2022
Publication statusPublished - 10 Jan 2023


  • DNA origami dimerization
  • DNA origami
  • DNA nanotechnology
  • fluorous DNA
  • self-assembly
  • molecular recognition
  • fluorous


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