Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Nicholas J. W. Rattray; Waleed A. Zalloum; David Mansell; Joe Latimer; Carl H. Schwalbe; Alexander J. Blake; Elena V. Bichenkova; Sally Freeman

doi:10.1039/C2CC32521G

Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Nicholas J. W. Rattray, Waleed A. Zalloum, David Mansell, Joe Latimer, Carl H. Schwalbe, Alexander J. Blake, Elena V. Bichenkova, Sally Freeman

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

Original language	English
Pages (from-to)	6393-6395
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	51
DOIs	https://doi.org/10.1039/C2CC32521G
Publication status	Published - 9 May 2012

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Keywords

molecular probe
pathogenic bacteria
infection

Cite this

Rattray, N. J. W., Zalloum, W. A., Mansell, D., Latimer, J., Schwalbe, C. H., Blake, A. J., ... Freeman, S. (2012). Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge. Chemical Communications, 48(51), 6393-6395. https://doi.org/10.1039/C2CC32521G

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abstract = "Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.",

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doi = "10.1039/C2CC32521G",

language = "English",

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Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge. / Rattray, Nicholas J. W.; Zalloum, Waleed A.; Mansell, David; Latimer, Joe; Schwalbe, Carl H.; Blake, Alexander J.; Bichenkova, Elena V.; Freeman, Sally.

In: Chemical Communications, Vol. 48, No. 51, 09.05.2012, p. 6393-6395.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

AU - Rattray, Nicholas J. W.

AU - Zalloum, Waleed A.

AU - Mansell, David

AU - Latimer, Joe

AU - Schwalbe, Carl H.

AU - Blake, Alexander J.

AU - Bichenkova, Elena V.

AU - Freeman, Sally

PY - 2012/5/9

Y1 - 2012/5/9

N2 - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

AB - Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.

KW - molecular probe

KW - pathogenic bacteria

KW - infection

U2 - 10.1039/C2CC32521G

DO - 10.1039/C2CC32521G

M3 - Article

VL - 48

SP - 6393

EP - 6395

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 51

ER -

Access to Document

10.1039/C2CC32521G