Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge

Nicholas J. W. Rattray, Waleed A. Zalloum, David Mansell, Joe Latimer, Carl H. Schwalbe, Alexander J. Blake, Elena V. Bichenkova, Sally Freeman

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Abstract

Using biomimetic chemical reduction or Clostridium perfringens cell extract containing azoreductase, the dimer-fluorescent probe 2,4-O-bisdansyl-6,7-diazabicyclooct-6-ene, which possesses a conformationally constrained cis-azo bridge, is reduced to the tetra-equatorial 2,4-O-bisdansyl-cyclohexyl-3,5-bisammonium salt which exhibits fluorescence indicative of a dansyl monomer.
Original languageEnglish
Pages (from-to)6393-6395
Number of pages3
JournalChemical Communications
Volume48
Issue number51
DOIs
Publication statusPublished - 9 May 2012

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Keywords

  • molecular probe
  • pathogenic bacteria
  • infection

Cite this

Rattray, N. J. W., Zalloum, W. A., Mansell, D., Latimer, J., Schwalbe, C. H., Blake, A. J., ... Freeman, S. (2012). Fluorescent probe for detection of bacteria: conformational trigger upon bacterial reduction of an azo bridge. Chemical Communications, 48(51), 6393-6395. https://doi.org/10.1039/C2CC32521G