Fluorescence resonance energy transfer from allophycocyanin to malachite green

O.J. Rolinski; D.J.S. Birch; L.J. McCartney; J.C. Pickup

doi:10.1016/S0009-2614(99)00707-1

Fluorescence resonance energy transfer from allophycocyanin to malachite green

O.J. Rolinski
, D.J.S. Birch
, L.J. McCartney
, J.C. Pickup

Physics

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.

Original language	English
Pages (from-to)	395-401
Number of pages	6
Journal	Chemical Physics Letters
Volume	309
Issue number	5-6
DOIs	https://doi.org/10.1016/S0009-2614(99)00707-1
Publication status	Published - 20 Aug 1999

Keywords

fluorescence resonance
energy transfer
allophycocyanin to malachite green

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10.1016/S0009-2614(99)00707-1

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title = "Fluorescence resonance energy transfer from allophycocyanin to malachite green",

abstract = "The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from F{\"o}rster decay kinetics. The use of APC in trans-dermal measurements is proposed.",

keywords = "fluorescence resonance, energy transfer, allophycocyanin to malachite green",

author = "O.J. Rolinski and D.J.S. Birch and L.J. McCartney and J.C. Pickup",

year = "1999",

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doi = "10.1016/S0009-2614(99)00707-1",

language = "English",

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pages = "395--401",

journal = "Chemical Physics Letters",

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TY - JOUR

T1 - Fluorescence resonance energy transfer from allophycocyanin to malachite green

AU - Rolinski, O.J.

AU - Birch, D.J.S.

AU - McCartney, L.J.

AU - Pickup, J.C.

PY - 1999/8/20

Y1 - 1999/8/20

N2 - The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.

AB - The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.

KW - fluorescence resonance

KW - energy transfer

KW - allophycocyanin to malachite green

UR - http://dx.doi.org/10.1016/S0009-2614(99)00707-1

U2 - 10.1016/S0009-2614(99)00707-1

DO - 10.1016/S0009-2614(99)00707-1

M3 - Article

SN - 0009-2614

VL - 309

SP - 395

EP - 401

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 5-6

ER -

Fluorescence resonance energy transfer from allophycocyanin to malachite green

Abstract

Keywords

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