Fluorescence resonance energy transfer from allophycocyanin to malachite green

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.
LanguageEnglish
Pages395-401
Number of pages6
JournalChemical Physics Letters
Volume309
Issue number5-6
DOIs
Publication statusPublished - 20 Aug 1999

Fingerprint

resonance fluorescence
energy transfer
Kinetics
kinetics
Phycobiliproteins
decay
complex systems
Large scale systems
Quenching
Fluorescence
quenching
Binding Sites
Infrared radiation
fluorescence
malachite green
allophycocyanin
Fluorescence Resonance Energy Transfer

Keywords

  • fluorescence resonance
  • energy transfer
  • allophycocyanin to malachite green

Cite this

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title = "Fluorescence resonance energy transfer from allophycocyanin to malachite green",
abstract = "The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from F{\"o}rster decay kinetics. The use of APC in trans-dermal measurements is proposed.",
keywords = "fluorescence resonance, energy transfer, allophycocyanin to malachite green",
author = "O.J. Rolinski and D.J.S. Birch and L.J. McCartney and J.C. Pickup",
year = "1999",
month = "8",
day = "20",
doi = "10.1016/S0009-2614(99)00707-1",
language = "English",
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journal = "Chemical Physics Letters",
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Fluorescence resonance energy transfer from allophycocyanin to malachite green. / Rolinski, O.J.; Birch, D.J.S.; McCartney, L.J.; Pickup, J.C.

In: Chemical Physics Letters, Vol. 309, No. 5-6, 20.08.1999, p. 395-401.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fluorescence resonance energy transfer from allophycocyanin to malachite green

AU - Rolinski, O.J.

AU - Birch, D.J.S.

AU - McCartney, L.J.

AU - Pickup, J.C.

PY - 1999/8/20

Y1 - 1999/8/20

N2 - The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.

AB - The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.

KW - fluorescence resonance

KW - energy transfer

KW - allophycocyanin to malachite green

UR - http://dx.doi.org/10.1016/S0009-2614(99)00707-1

U2 - 10.1016/S0009-2614(99)00707-1

DO - 10.1016/S0009-2614(99)00707-1

M3 - Article

VL - 309

SP - 395

EP - 401

JO - Chemical Physics Letters

T2 - Chemical Physics Letters

JF - Chemical Physics Letters

SN - 0009-2614

IS - 5-6

ER -