Fluorescence resonance energy transfer from allophycocyanin to malachite green

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Abstract

The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.
Original languageEnglish
Pages (from-to)395-401
Number of pages6
JournalChemical Physics Letters
Volume309
Issue number5-6
DOIs
Publication statusPublished - 20 Aug 1999

Keywords

  • fluorescence resonance
  • energy transfer
  • allophycocyanin to malachite green

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