The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Förster decay kinetics. The use of APC in trans-dermal measurements is proposed.
- fluorescence resonance
- energy transfer
- allophycocyanin to malachite green