Fluorescence nanotomography using resonance energy transfer: Demonstration with a protein-sugar complex

Research output: Contribution to journalArticle

Abstract

A new approach to structural sensing, fluorescence resonance energy transfer nanotomography, which interprets fluorescence decay measurement in terms of site density analysis of molecular distributions, has been applied to a glucose sensor based on competitive binding with malachite green labelled dextran to the sugar binding protein concanavalinA labelled with allophycocyanin. Opportunities for structural sensing in clinical medicine are highlighted.
Original languageEnglish
Pages (from-to)N221-N226
JournalPhysics in Medicine and Biology
Volume46
Issue number2001
DOIs
Publication statusPublished - 22 Aug 2001

Keywords

  • fluorescence nanotomography
  • resonance energy transfer
  • protein-sugar complex
  • nanoscience

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