Fluorescence nanotomography using resonance energy transfer: Demonstration with a protein-sugar complex

Olaf J. Rolinski, David J.S. Birch, Lydia J. McCartney, John C. Pickup

Research output: Contribution to journalArticlepeer-review


A new approach to structural sensing, fluorescence resonance energy transfer nanotomography, which interprets fluorescence decay measurement in terms of site density analysis of molecular distributions, has been applied to a glucose sensor based on competitive binding with malachite green labelled dextran to the sugar binding protein concanavalinA labelled with allophycocyanin. Opportunities for structural sensing in clinical medicine are highlighted.
Original languageEnglish
Pages (from-to)N221-N226
JournalPhysics in Medicine and Biology
Issue number2001
Publication statusPublished - 22 Aug 2001


  • fluorescence nanotomography
  • resonance energy transfer
  • protein-sugar complex
  • nanoscience

Cite this