Fluorescence nanotomography: a structural tool in biomedical sensing

Research output: Contribution to conferencePaper

Abstract

Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.
Original languageEnglish
Pages836-847
Number of pages11
Publication statusPublished - 2003
EventOpto-Ireland 2002: Optics and Photonics Technologies and Applications - Galway, Ireland
Duration: 5 Sep 2002 → …

Conference

ConferenceOpto-Ireland 2002: Optics and Photonics Technologies and Applications
CityGalway, Ireland
Period5/09/02 → …

Keywords

  • fluorescence sensors
  • FRET
  • fluorescence lifetime measurement

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    Rolinski, O. J., & Birch, D. J. S. (2003). Fluorescence nanotomography: a structural tool in biomedical sensing. 836-847. Paper presented at Opto-Ireland 2002: Optics and Photonics Technologies and Applications, Galway, Ireland, .