Abstract
| Language | English |
|---|---|
| Pages | 836-847 |
| Number of pages | 11 |
| Publication status | Published - 2003 |
| Event | Opto-Ireland 2002: Optics and Photonics Technologies and Applications - Galway, Ireland Duration: 5 Sep 2002 → … |
Conference
| Conference | Opto-Ireland 2002: Optics and Photonics Technologies and Applications |
|---|---|
| City | Galway, Ireland |
| Period | 5/09/02 → … |
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Keywords
- fluorescence sensors
- FRET
- fluorescence lifetime measurement
Cite this
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Fluorescence nanotomography: a structural tool in biomedical sensing. / Rolinski, O.J.; Birch, D.J.S.
2003. 836-847 Paper presented at Opto-Ireland 2002: Optics and Photonics Technologies and Applications, Galway, Ireland, .Research output: Contribution to conference › Paper
TY - CONF
T1 - Fluorescence nanotomography: a structural tool in biomedical sensing
AU - Rolinski, O.J.
AU - Birch, D.J.S.
PY - 2003
Y1 - 2003
N2 - Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.
AB - Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.
KW - fluorescence sensors
KW - FRET
KW - fluorescence lifetime measurement
UR - http://dx.doi.org/10.1117/12.463935
M3 - Paper
SP - 836
EP - 847
ER -