Fluorescence nanotomography: a structural tool in biomedical sensing

Research output: Contribution to conferencePaper

Abstract

Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.
LanguageEnglish
Pages836-847
Number of pages11
Publication statusPublished - 2003
EventOpto-Ireland 2002: Optics and Photonics Technologies and Applications - Galway, Ireland
Duration: 5 Sep 2002 → …

Conference

ConferenceOpto-Ireland 2002: Optics and Photonics Technologies and Applications
CityGalway, Ireland
Period5/09/02 → …

Fingerprint

Fluorescence
Glucose sensors
Molecules
Macromolecules
Sugars
Distribution functions
Phospholipids
Polymers
Demonstrations
Proteins

Keywords

  • fluorescence sensors
  • FRET
  • fluorescence lifetime measurement

Cite this

Rolinski, O. J., & Birch, D. J. S. (2003). Fluorescence nanotomography: a structural tool in biomedical sensing. 836-847. Paper presented at Opto-Ireland 2002: Optics and Photonics Technologies and Applications, Galway, Ireland, .
Rolinski, O.J. ; Birch, D.J.S. / Fluorescence nanotomography: a structural tool in biomedical sensing. Paper presented at Opto-Ireland 2002: Optics and Photonics Technologies and Applications, Galway, Ireland, .11 p.
@conference{779269a332554cc696f7f29ceaadf355,
title = "Fluorescence nanotomography: a structural tool in biomedical sensing",
abstract = "Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.",
keywords = "fluorescence sensors, FRET, fluorescence lifetime measurement",
author = "O.J. Rolinski and D.J.S. Birch",
year = "2003",
language = "English",
pages = "836--847",
note = "Opto-Ireland 2002: Optics and Photonics Technologies and Applications ; Conference date: 05-09-2002",

}

Rolinski, OJ & Birch, DJS 2003, 'Fluorescence nanotomography: a structural tool in biomedical sensing' Paper presented at Opto-Ireland 2002: Optics and Photonics Technologies and Applications, Galway, Ireland, 5/09/02, pp. 836-847.

Fluorescence nanotomography: a structural tool in biomedical sensing. / Rolinski, O.J.; Birch, D.J.S.

2003. 836-847 Paper presented at Opto-Ireland 2002: Optics and Photonics Technologies and Applications, Galway, Ireland, .

Research output: Contribution to conferencePaper

TY - CONF

T1 - Fluorescence nanotomography: a structural tool in biomedical sensing

AU - Rolinski, O.J.

AU - Birch, D.J.S.

PY - 2003

Y1 - 2003

N2 - Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.

AB - Fluorescence nanotomography (FN) is a newly developed method for determining molecular distributions on a nanometre scale in soft solids, biological macromolecules and medically important systems. FN uses fluorescence resonance energy transfer (FRET) for the recognition of the separations between molecules. By using a fluorescence lifetime measurement of sub-nanosecond time resolution, the spatial resolution of the resulting distribution function can be better than 1 �. In this paper the theoretical background of the method is outlined and the results of simulations on model molecular distributions presented. This is followed by demonstration of several applications of FN to real molecular systems, including bulk solutions of molecules of different sizes, complexes, porous polymers, phospholipids and sugar-protein competitive binding sensors glucose. The experimental requirements of FN as a structural tool for wide class of biomedical systems are discussed.

KW - fluorescence sensors

KW - FRET

KW - fluorescence lifetime measurement

UR - http://dx.doi.org/10.1117/12.463935

M3 - Paper

SP - 836

EP - 847

ER -

Rolinski OJ, Birch DJS. Fluorescence nanotomography: a structural tool in biomedical sensing. 2003. Paper presented at Opto-Ireland 2002: Optics and Photonics Technologies and Applications, Galway, Ireland, .