Fluorescence lifetime estimation method for incomplete decay

Hongqi Yu, David Day-Uei Li

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1 Citation (Scopus)
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A new incomplete decay signal model is proposed to describe the
incomplete decay effects in a time-correlated single-photon counting
(TCSPC) based fluorescence lifetime imaging (FLIM) system. Based on
this model, we modified a MUltiple SIgnal Classification (MUSIC)
algorithm to eliminate the incomplete decay effects. Monte Carlo
simulations were carried out to demonstrate the performances of the
proposed approach. Simulations show that the proposed method is
insensitive to the laser pulse rate and has a larger lifetime dynamic range
compared with previously reported approaches. As far as we know, this
new method is the first non-fitting method that can resolve incomplete
decay effects for multi-exponential decays.
Original languageEnglish
Number of pages2
JournalElectronics Letters
Early online date25 Sept 2015
Publication statusPublished - 25 Sept 2015


  • decay signal model
  • decay effects
  • fluorescence lifetime imaging


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