Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager

Gerard Giraud, Holger Schulze, Day-Uei Li, Till T. Bachmann, Jason Crain, David Tyndall, Justin Richardson, Richard Walker, David Stoppa, Edoardo Charbon, Robert Henderson, Jochen Arlt

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Fluorescence lifetime of dye molecules is a sensitive reporter on local microenvironment which is generally independent of fluorophores concentration and can be used as a means of discrimination between molecules with spectrally overlapping emission. It is therefore a potentially powerful multiplexed detection modality in biosensing but requires extremely low light level operation typical of biological analyte concentrations, long data acquisition periods and on-chip processing capability to realize these advantages. We report here fluorescence lifetime data obtained using a CMOS-SPAD imager in conjunction with DNA microarrays and TIRF excitation geometry. This enables acquisition of single photon arrival time histograms for a 320 pixel FLIM map within less than 26 seconds exposure time. From this, we resolve distinct lifetime signatures corresponding to dye-labelled HCV and quantum-dot-labelled HCMV nucleic acid targets at concentrations as low as 10 nM.
LanguageEnglish
Pages1302-1308
Number of pages7
JournalBiomedical Optics Express
DOIs
Publication statusPublished - 4 Nov 2010

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Microarrays
Oligonucleotide Array Sequence Analysis
Image sensors
CMOS
DNA
Coloring Agents
Dyes
deoxyribonucleic acid
Fluorescence
Quantum Dots
life (durability)
fluorescence
Molecules
Fluorophores
Nucleic acids
dyes
Photons
Nucleic Acids
Semiconductor quantum dots
Data acquisition

Keywords

  • fluorescence microscopy
  • lifetime-based sensing
  • medical optics instrumentation
  • biological sensing and sensors
  • avalanche photodiodes

Cite this

Giraud, G., Schulze, H., Li, D-U., Bachmann, T. T., Crain, J., Tyndall, D., ... Arlt, J. (2010). Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager. Biomedical Optics Express, 1302-1308. https://doi.org/10.1364/BOE.1.001302
Giraud, Gerard ; Schulze, Holger ; Li, Day-Uei ; Bachmann, Till T. ; Crain, Jason ; Tyndall, David ; Richardson, Justin ; Walker, Richard ; Stoppa, David ; Charbon, Edoardo ; Henderson, Robert ; Arlt, Jochen. / Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager. In: Biomedical Optics Express. 2010 ; pp. 1302-1308.
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Giraud, G, Schulze, H, Li, D-U, Bachmann, TT, Crain, J, Tyndall, D, Richardson, J, Walker, R, Stoppa, D, Charbon, E, Henderson, R & Arlt, J 2010, 'Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager' Biomedical Optics Express, pp. 1302-1308. https://doi.org/10.1364/BOE.1.001302

Fluorescence lifetime biosensing with DNA microarrays and a CMOS-SPAD imager. / Giraud, Gerard; Schulze, Holger; Li, Day-Uei; Bachmann, Till T.; Crain, Jason; Tyndall, David; Richardson, Justin; Walker, Richard; Stoppa, David; Charbon, Edoardo; Henderson, Robert; Arlt, Jochen.

In: Biomedical Optics Express, 04.11.2010, p. 1302-1308.

Research output: Contribution to journalArticle

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AU - Crain, Jason

AU - Tyndall, David

AU - Richardson, Justin

AU - Walker, Richard

AU - Stoppa, David

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AU - Henderson, Robert

AU - Arlt, Jochen

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