Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein

John  Pickup; Faaizah  Khan; Zheng-Liang Zhi; Jonathan Coulter; David J S Birch

doi:10.1177/193229681300700108

Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein

John Pickup, Faaizah Khan, Zheng-Liang Zhi, Jonathan Coulter, David J S Birch

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

We review recent progress in our laboratories towards developing in vivo glucose sensors for diabetes that are based on fluorescence labelling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labelling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber optic-based glucose sensor using this technology is being tested.

Original language	English
Pages (from-to)	62-71
Number of pages	10
Journal	Journal of Diabetes Science and Technology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1177/193229681300700108
Publication status	Published - Jan 2013

Keywords

diabetes
continuous glucose monitoring
glucose sensor
glucose/galactose binding protein
fluorescence

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/193229681300700108

Cite this

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abstract = "We review recent progress in our laboratories towards developing in vivo glucose sensors for diabetes that are based on fluorescence labelling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labelling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber optic-based glucose sensor using this technology is being tested.",

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AU - Birch, David J S

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AB - We review recent progress in our laboratories towards developing in vivo glucose sensors for diabetes that are based on fluorescence labelling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labelling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber optic-based glucose sensor using this technology is being tested.

KW - diabetes

KW - continuous glucose monitoring

KW - glucose sensor

KW - glucose/galactose binding protein

KW - fluorescence

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692217/

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Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein

Abstract

Keywords

UN SDGs

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