Fluorescence-based glucose sensors

J.C. Pickup, Faeiza Hussain, Nicholas D. Evans, O.J. Rolinski, D.J.S. Birch

Research output: Contribution to journalArticle

419 Citations (Scopus)

Abstract

There is an urgent need to develop technology for continuous in vivo glucose monitoring in subjects with diabetes mellitus. Problems with existing devices based on electrochemistry have encouraged alternative approaches to glucose sensing in recent years, and those based on fluorescence intensity and lifetime have special advantages, including sensitivity and the potential for non-invasive measurement when near-infrared light is used. Several receptors have been employed to detect glucose in fluorescence sensors, and these include the lectin concanavalin A (Con A), enzymes such as glucose oxidase, glucose dehydrogenase and hexokinase/glucokinase, bacterial glucose-binding protein, and boronic acid derivatives (which bind the diols of sugars). Techniques include measuring changes in fluorescence resonance energy transfer (FRET) between a fluorescent donor and an acceptor either within a protein which undergoes glucose-induced changes in conformation or because of competitive displacement; measurement of glucose-induced changes in intrinsic fluorescence of enzymes (e.g. due to tryptophan residues in hexokinase) or extrinsic fluorophores (e.g. using environmentally sensitive fluorophores to signal protein conformation). Non-invasive glucose monitoring can be accomplished by measurement of cell autofluorescence due to NAD(P)H, and fluorescent markers of mitochondrial metabolism can signal changes in extracellular glucose concentration. Here we review the principles of operation, context and current status of the various approaches to fluorescence-based glucose sensing.
Original languageEnglish
Pages (from-to)2555-2565
Number of pages10
JournalBiosensors and Bioelectronics
Volume20
Issue number12
DOIs
Publication statusPublished - 15 Jun 2005

Fingerprint

Glucose sensors
Glucose
Fluorescence
Hexokinase
Fluorophores
Conformations
Glucose 1-Dehydrogenase
Boronic Acids
Enzymes
Electrochemistry
Glucokinase
Glucose Oxidase
Fluorescence Resonance Energy Transfer
Protein Conformation
Proteins
Monitoring
Glucose oxidase
Medical problems
Concanavalin A
Lectins

Keywords

  • diabetes mellitus
  • biosensor
  • glucose monitoring
  • glucose sensor
  • fluorescence
  • non-invasive monitoring
  • fluorescence resonance energy transfer

Cite this

Pickup, J.C. ; Hussain, Faeiza ; Evans, Nicholas D. ; Rolinski, O.J. ; Birch, D.J.S. / Fluorescence-based glucose sensors. In: Biosensors and Bioelectronics. 2005 ; Vol. 20, No. 12. pp. 2555-2565.
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Fluorescence-based glucose sensors. / Pickup, J.C.; Hussain, Faeiza; Evans, Nicholas D.; Rolinski, O.J.; Birch, D.J.S.

In: Biosensors and Bioelectronics, Vol. 20, No. 12, 15.06.2005, p. 2555-2565.

Research output: Contribution to journalArticle

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