Fluorescence anisotropy of protein - gold nanoclusters

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

3 Citations (Scopus)

Abstract

Red-emitting gold nanoclusters (BSA-Au-25) have been synthesized in Bovine Serum albumin and found to have a characteristic fluorescence lifetime of 1.25 mu s. Fluorescence anisotropy measurements of BSA-Au-25 reveal a dramatic increase in protein size as the pH is reduced from 7 to 3.1, consistent with the change of BSA from N form to E form. This study suggests the possibility of exploiting fluorescent protein-gold nanoclusters to probe protein conformational changes in response to the local environment.
Original languageEnglish
Title of host publication2012 12th IEEE Conference on Nanotechnology
Subtitle of host publicationIEEE Nano
Place of PublicationNew York
PublisherIEEE
Number of pages4
ISBN (Print)9781467322003
DOIs
Publication statusPublished - 2012

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Keywords

  • gold nanoclusters
  • fluorescence anisotropy
  • BSA
  • protein conformational change
  • color
  • proteins
  • probes
  • green products
  • gold
  • fluorescence
  • detectors

Cite this

Li, C., Sutter, J., Birch, D., & Chen, Y. (2012). Fluorescence anisotropy of protein - gold nanoclusters. In 2012 12th IEEE Conference on Nanotechnology: IEEE Nano New York: IEEE. https://doi.org/10.1109/NANO.2012.6322067