Abstract
Red-emitting gold nanoclusters (BSA-Au-25) have been synthesized in Bovine Serum albumin and found to have a characteristic fluorescence lifetime of 1.25 mu s. Fluorescence anisotropy measurements of BSA-Au-25 reveal a dramatic increase in protein size as the pH is reduced from 7 to 3.1, consistent with the change of BSA from N form to E form. This study suggests the possibility of exploiting fluorescent protein-gold nanoclusters to probe protein conformational changes in response to the local environment.
| Original language | English |
|---|---|
| Title of host publication | 2012 12th IEEE Conference on Nanotechnology |
| Subtitle of host publication | IEEE Nano |
| Place of Publication | New York |
| Publisher | IEEE |
| Number of pages | 4 |
| ISBN (Print) | 9781467322003 |
| DOIs | |
| Publication status | Published - 2012 |
Keywords
- gold nanoclusters
- fluorescence anisotropy
- BSA
- protein conformational change
- color
- proteins
- probes
- green products
- gold
- fluorescence
- detectors
