Red-emitting gold nanoclusters (BSA-Au-25) have been synthesized in Bovine Serum albumin and found to have a characteristic fluorescence lifetime of 1.25 mu s. Fluorescence anisotropy measurements of BSA-Au-25 reveal a dramatic increase in protein size as the pH is reduced from 7 to 3.1, consistent with the change of BSA from N form to E form. This study suggests the possibility of exploiting fluorescent protein-gold nanoclusters to probe protein conformational changes in response to the local environment.
- gold nanoclusters
- fluorescence anisotropy
- protein conformational change
- green products
Li, C., Sutter, J., Birch, D., & Chen, Y. (2012). Fluorescence anisotropy of protein - gold nanoclusters. In 2012 12th IEEE Conference on Nanotechnology: IEEE Nano New York: IEEE. https://doi.org/10.1109/NANO.2012.6322067