Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension

Susan Chalmers, Christopher D. Saunter, John M. Girkin, John G. McCarron

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mitochondrial morphology is central to normal physiology and disease development. However, in many live cells and tissues, complex mitochondrial structures exist and morphology has been difficult to quantify. We have measured the shape of electrically-discrete mitochondria, imaging them individually to restore detail hidden in clusters and demarcate functional boundaries. Stochastic "flickers" of mitochondrial membrane potential were visualized with a rapidly-partitioning fluorophore and the pixel-by-pixel covariance of spatio-temporal fluorescence changes analyzed. This Flicker-assisted Localization Microscopy (FaLM) requires only an epifluorescence microscope and sensitive camera. In vascular myocytes, the apparent variation in mitochondrial size was partly explained by densely-packed small mitochondria. In normotensive animals, mitochondria were small spheres or rods. In hypertension, mitochondria were larger, occupied more of the cell volume and were more densely clustered. FaLM provides a convenient tool for increased discrimination of mitochondrial architecture and has revealed mitochondrial alterations that may contribute to hypertension.
LanguageEnglish
Article number16875
Number of pages15
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 23 Nov 2015

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Microscopy
Mitochondria
Hypertension
Mitochondrial Size
Mitochondrial Membrane Potential
Cell Size
Muscle Cells
Blood Vessels
Fluorescence

Keywords

  • mitochondrial morphology
  • flicker-assisted localization microscopy
  • hypertension

Cite this

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Flicker-assisted localization microscopy reveals altered mitochondrial architecture in hypertension. / Chalmers, Susan; Saunter, Christopher D.; Girkin, John M.; McCarron, John G.

In: Scientific Reports, Vol. 5, 16875, 23.11.2015.

Research output: Contribution to journalArticle

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