Fingerprinting polychlorinated biphenyls in environmental samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry

David Megson, Robert Kalin, Paul J Worsfold, Caroline Gauchotte-Lindsay, Donald G Patterson, Maeve C Lohan, Sean Comber, Thomas A Brown, Gwen O' Sullivan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A GC × GC-TOFMS installed with a Rtx-PCB (60 m × 0.18 mm × 0.18 μm) in the first dimension and Rxi- 17 (1.5 m × 0.1 mm × 0.1 μm) column in the second dimension was used to separate 188 out of 209 congeners. A further 12 congeners were identified through additional data processing resulting in the identification of a total of 200 congeners. However, caution is advised if these 12 congeners were to be used in quantitative assessments. The remaining 9 co-eluting congeners were three doublets (CB65 + CB62, CB160 + CB163 and CB201 + CB204) and one triplet (CB20 + CB21 + CB33). This method was tested on five Aroclors and resulted in the separation of all congeners present in the heavier Aroclor mixtures A1254 and A1260. The suitability of this method for applications in biological matrices was demonstrated on extracted whiting and guillemot liver samples which resulted in the identification of 137 individual PCBs in the whiting liver sample and 120 in the guillemot sample. Fingerprinting was able to show clear differences in the PCB signature of the two animals. This highlights the potential of this method for PCB fingerprinting in environmental forensics studies and other assessments that require congener specific analysis.

LanguageEnglish
Pages276-283
Number of pages8
JournalJournal of Chromatography A
Volume1318
DOIs
Publication statusPublished - 29 Nov 2013

Fingerprint

Polychlorinated Biphenyls
Polychlorinated biphenyls
Gas chromatography
Gas Chromatography
Mass spectrometry
Mass Spectrometry
Aroclors
Liver
Animals

Keywords

  • environmental pollutants
  • gas chromatography-mass spectrometry
  • polychlorinated biphenyls
  • stereoisomerism

Cite this

Megson, David ; Kalin, Robert ; Worsfold, Paul J ; Gauchotte-Lindsay, Caroline ; Patterson, Donald G ; Lohan, Maeve C ; Comber, Sean ; Brown, Thomas A ; O' Sullivan, Gwen. / Fingerprinting polychlorinated biphenyls in environmental samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry. In: Journal of Chromatography A . 2013 ; Vol. 1318. pp. 276-283.
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Fingerprinting polychlorinated biphenyls in environmental samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry. / Megson, David; Kalin, Robert; Worsfold, Paul J; Gauchotte-Lindsay, Caroline; Patterson, Donald G; Lohan, Maeve C; Comber, Sean; Brown, Thomas A; O' Sullivan, Gwen.

In: Journal of Chromatography A , Vol. 1318, 29.11.2013, p. 276-283.

Research output: Contribution to journalArticle

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AU - Megson, David

AU - Kalin, Robert

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AU - Gauchotte-Lindsay, Caroline

AU - Patterson, Donald G

AU - Lohan, Maeve C

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AU - Brown, Thomas A

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