Fiber photometry-based investigation of brain function and dysfunction

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Fiber photometry is an optical method to monitor fluorescent signals using a fiber optic cannula. Over the past two decades, together with the development of various genetically encoded biosensors, it has been applied to investigate various types of activity in the central nervous system. This includes not only type-specific neuronal population activity, but also non-neuronal activity and neurotransmitter/neuropeptide signals in awake, freely behaving animals. In this perspective, we summarize the recent development of this technique. After describing common technical pitfalls, we discuss future directions of this powerful approach for investigating brain function and dysfunction.
Original languageEnglish
Article numberS11502
Number of pages7
Issue numberS1
Publication statusPublished - 14 Nov 2023


  • fiber photometry
  • calcium imaging
  • cell type
  • astrocyte
  • neural oscillation


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