Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance

Suhee Kim, Alastair W. Wark, Hye Jin Lee

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)
134 Downloads (Pure)

Abstract

The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer’s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.
Original languageEnglish
Pages (from-to)7793-7799
Number of pages7
JournalAnalytical Chemistry
Volume88
Issue number15
DOIs
Publication statusPublished - 11 Jul 2016

Keywords

  • Tau protein
  • biomarkers
  • Alzheimer's disease
  • neurodegenerative screening

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