Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance

Suhee Kim; Alastair W. Wark; Hye Jin Lee

doi:10.1021/acs.analchem.6b01825

Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance

Suhee Kim, Alastair W. Wark, Hye Jin Lee

Pure And Applied Chemistry

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78 Citations (Scopus)

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Abstract

The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer’s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.

Original language	English
Pages (from-to)	7793-7799
Number of pages	7
Journal	Analytical Chemistry
Volume	88
Issue number	15
DOIs	https://doi.org/10.1021/acs.analchem.6b01825
Publication status	Published - 11 Jul 2016

Keywords

Tau protein
biomarkers
Alzheimer's disease
neurodegenerative screening

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.analchem.6b01825

Kim-Wark-Lee-PAC-2016-femtomolar-detection-of-tau-proteins-in-undiluted-plasmaAccepted author manuscript, 1.3 MB

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title = "Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance",

abstract = "The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer{\textquoteright}s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.",

keywords = "Tau protein, biomarkers, Alzheimer's disease, neurodegenerative screening",

author = "Suhee Kim and Wark, {Alastair W.} and Lee, {Hye Jin}",

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AU - Kim, Suhee

AU - Wark, Alastair W.

AU - Lee, Hye Jin

N1 - This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Analytical Chemistry, copyright © American Chemical Society after peer review. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b01825

PY - 2016/7/11

Y1 - 2016/7/11

N2 - The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer’s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.

AB - The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer’s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.

KW - Tau protein

KW - biomarkers

KW - Alzheimer's disease

KW - neurodegenerative screening

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EP - 7799

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 15

ER -

Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance

Abstract

Keywords

UN SDGs

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