Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance

Suhee Kim, Alastair W. Wark, Hye Jin Lee

Research output: Contribution to journalArticle

31 Citations (Scopus)
114 Downloads (Pure)

Abstract

The ability to directly detect Tau protein and other neurodegenerative biomarkers in human plasma at clinically relevant concentrations continues to be a significant hurdle for the establishment of diagnostic tests for Alzheimer’s disease (AD). In this article, we introduce a new DNA aptamer/antibody sandwich assay pairing and apply it for the detection of human Tau 381 in undiluted plasma at concentrations as low as 10 fM. This was achieved on a multichannel surface plasmon resonance (SPR) platform with the challenge of working in plasma overcome through the development of a tailored mixed monolayer surface chemistry. In addition, a robust methodology was developed involving various same chip control measurements on reference channels to which the detection signal was normalized. Comparative measurements in plasma between SPR and enzyme-linked immunosorbent assay (ELISA) measurements were also performed to highlight both the 1000-fold performance enhancement of SPR and the ability to measure both spiked and native concentrations that are not achievable with ELISA.
Original languageEnglish
Pages (from-to)7793-7799
Number of pages7
JournalAnalytical Chemistry
Volume88
Issue number15
DOIs
Publication statusPublished - 11 Jul 2016

Keywords

  • Tau protein
  • biomarkers
  • Alzheimer's disease
  • neurodegenerative screening

Fingerprint Dive into the research topics of 'Femtomolar detection of Tau proteins in undiluted plasma using surface plasmon resonance'. Together they form a unique fingerprint.

  • Cite this