Fate of tetracycline resistance genes in aquatic systems

migration from the water column to peripheral biofilms

Christina A. Engemann, Patricia L. Keen, Charles W. Knapp, Kenneth J. Hall, David W. Graham

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Antibiotic resistance genes (ARGs) are emerging contaminants that are being found at elevated levels in sediments and other aquatic compartments in areas of intensive agricultural and urban activity. However, little quantitative data exist on the migration and attenuation of ARGs in natural ecosystems, which is central to predicting their fate after release into receiving waters. Here we examined the fate of tetra cycline-resistance genes in bacterial hosts released in cattle feedlot wastewater using field-scale mesocosms to quantify ARG attenuation rate in the water column and also the migration of ARGs into peripheral biofilms. Feedlot wastewater was added to fifteen cylindrical 11.3-m(3) mesocosms (some of which had artificial substrates) simulating five different receiving water conditions (in triplicate), and the abundance of six resistance genes (tet(O), tet(W) tet(M), tet(Q), tet(B), and tet(L) and 16S-rRNA genes was monitored for 14 days. Mesocosm treatments were varied according to light supply, microbial supplements (via river water additions), and oxytetracycline (OTC) level. First-order water column disappearance coefficients (k(d)) for the sum of the six genes (tetR) were always higher in sunlight than in the dark (-0.72 d(-1) and -0.51 d(-1), respectively). However, water column kd varied among genes (tet(O) < tet(W) < tet(M) < tet(Q); tet(B) and tet(L) were below detection) and some genes, particularly tet(W), readily migrated into biofilms, suggesting that different genes be considered separately and peripheral compartments be included in future fate models. This work provides the first quantitative field data for modeling ARG fate in aquatic systems.
Original languageEnglish
Pages (from-to)5131-5136
Number of pages6
JournalEnvironmental Science and Technology
Volume42
Issue number14
Early online date6 Jun 2008
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Biofilms
Tetracycline
biofilm
Genes
water column
Water
gene
antibiotic resistance
Anti-Bacterial Agents
Wastewater
wastewater
artificial substrate
Oxytetracycline
oxytetracycline
mesocosm
Ecosystems
river water
cattle
Sediments
Rivers

Keywords

  • tetracycline resistance genes
  • aquatic asstems
  • migration
  • water column
  • peripheral biofilms

Cite this

Engemann, Christina A. ; Keen, Patricia L. ; Knapp, Charles W. ; Hall, Kenneth J. ; Graham, David W. / Fate of tetracycline resistance genes in aquatic systems : migration from the water column to peripheral biofilms. In: Environmental Science and Technology. 2008 ; Vol. 42, No. 14. pp. 5131-5136.
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abstract = "Antibiotic resistance genes (ARGs) are emerging contaminants that are being found at elevated levels in sediments and other aquatic compartments in areas of intensive agricultural and urban activity. However, little quantitative data exist on the migration and attenuation of ARGs in natural ecosystems, which is central to predicting their fate after release into receiving waters. Here we examined the fate of tetra cycline-resistance genes in bacterial hosts released in cattle feedlot wastewater using field-scale mesocosms to quantify ARG attenuation rate in the water column and also the migration of ARGs into peripheral biofilms. Feedlot wastewater was added to fifteen cylindrical 11.3-m(3) mesocosms (some of which had artificial substrates) simulating five different receiving water conditions (in triplicate), and the abundance of six resistance genes (tet(O), tet(W) tet(M), tet(Q), tet(B), and tet(L) and 16S-rRNA genes was monitored for 14 days. Mesocosm treatments were varied according to light supply, microbial supplements (via river water additions), and oxytetracycline (OTC) level. First-order water column disappearance coefficients (k(d)) for the sum of the six genes (tetR) were always higher in sunlight than in the dark (-0.72 d(-1) and -0.51 d(-1), respectively). However, water column kd varied among genes (tet(O) < tet(W) < tet(M) < tet(Q); tet(B) and tet(L) were below detection) and some genes, particularly tet(W), readily migrated into biofilms, suggesting that different genes be considered separately and peripheral compartments be included in future fate models. This work provides the first quantitative field data for modeling ARG fate in aquatic systems.",
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Fate of tetracycline resistance genes in aquatic systems : migration from the water column to peripheral biofilms. / Engemann, Christina A.; Keen, Patricia L.; Knapp, Charles W.; Hall, Kenneth J.; Graham, David W.

In: Environmental Science and Technology, Vol. 42, No. 14, 07.2008, p. 5131-5136.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fate of tetracycline resistance genes in aquatic systems

T2 - migration from the water column to peripheral biofilms

AU - Engemann, Christina A.

AU - Keen, Patricia L.

AU - Knapp, Charles W.

AU - Hall, Kenneth J.

AU - Graham, David W.

PY - 2008/7

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KW - tetracycline resistance genes

KW - aquatic asstems

KW - migration

KW - water column

KW - peripheral biofilms

UR - http://pubs.acs.org/doi/abs/10.1021/es800238e

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DO - 10.1021/es800238e

M3 - Article

VL - 42

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SN - 0013-936X

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