Fast wavelength-tunable ultra-violet laser source for confocal Fura-2AM imaging

E. Esposito, J. Harris, G. McConnell

Research output: Contribution to journalArticle

2 Citations (Scopus)
161 Downloads (Pure)

Abstract

We report a novel wavelength-flexible laser source for three-dimensional ultra-violet imaging. Based on supercontinuum generation in photonic crystal fiber, the resultant broadband laser source extended from A = 331 nm into the visible region of the spectrum. Using an electronically-controlled filter wheel and filter set with a response time of approximately 50 ins, rapid wavelength selection was performed. The described scheme is capable of exciting the current range of ultra-violet-excited fluorophores and the simple and rapid wavelength control also provides a new approach for fast ratiometric imaging of Fura-2AM, facilitating an easy method of performing quantitative intracellular calcium concentration measurements.

Original languageEnglish
Pages (from-to)470-473
Number of pages4
JournalOptics Communications
Volume270
Issue number2
DOIs
Publication statusPublished - 15 Feb 2007

Keywords

  • microscopy
  • cells
  • ultra-violet laser
  • imaging
  • frequency conversion

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