We report on the use of a computer-controlled digital micromirror device (DMD) to select discrete wavelength ranges from a white-light supercontinuum and the application of the light source to confocal laser scanning microscopy (CLSM). The fast switching rate of the DMD enabled video-rate wavelength tuning, while the high resolution allowed precise control over the bandwidth selection. The DMD system was used in conjunction with a CLSM system to characterize the wavelength switching capability, prior to performing three-dimensional CLSM of a variety of multiple-labeled samples in order to demonstrate the efficacy and practical application of the system.
- supercontinuum generation
- optical microscopy
- optical tuning
- optical switches
McConnell, G., Poland, S. P., & Girkin, J. M. (2006). Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy. Review of Scientific Instruments, 77(013702), 013702. https://doi.org/10.1063/1.2148996