Fast wavelength multiplexing of a white-light supercontinuum using a digital micromirror device for improved three-dimensional fluorescence microscopy

G. McConnell, S.P. Poland, J.M. Girkin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report on the use of a computer-controlled digital micromirror device (DMD) to select discrete wavelength ranges from a white-light supercontinuum and the application of the light source to confocal laser scanning microscopy (CLSM). The fast switching rate of the DMD enabled video-rate wavelength tuning, while the high resolution allowed precise control over the bandwidth selection. The DMD system was used in conjunction with a CLSM system to characterize the wavelength switching capability, prior to performing three-dimensional CLSM of a variety of multiple-labeled samples in order to demonstrate the efficacy and practical application of the system.
Original languageEnglish
Pages (from-to)013702
JournalReview of Scientific Instruments
Volume77
Issue number013702
DOIs
Publication statusPublished - 2006

Keywords

  • multiplexing
  • supercontinuum generation
  • micromirrors
  • fluorescence
  • optical microscopy
  • optical tuning
  • optical switches
  • photonics
  • biophotonics

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