Fast bi-exponential fluorescence lifetime imaging analysis methods

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A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.
Original languageEnglish
Pages (from-to)336-339
Number of pages4
JournalOptics Letters
Issue number3
Early online date21 Jan 2015
Publication statusPublished - 1 Feb 2015


  • coherence and statistical optics
  • photon counting
  • medical optics and biotechnology
  • fluorescence lifetime imaging
  • FLIM
  • time-correlated singlephoton counting


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