Fast bi-exponential fluorescence lifetime imaging analysis methods

David Day-Uei Li; Hongqi Yu; Yu Chen

doi:10.1364/OL.40.000336

Fast bi-exponential fluorescence lifetime imaging analysis methods

David Day-Uei Li, Hongqi Yu, Yu Chen

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

59 Downloads (Pure)

Abstract

A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.

Original language	English
Pages (from-to)	336-339
Number of pages	4
Journal	Optics Letters
Volume	40
Issue number	3
Early online date	21 Jan 2015
DOIs	https://doi.org/10.1364/OL.40.000336
Publication status	Published - 1 Feb 2015

Keywords

coherence and statistical optics
photon counting
medical optics and biotechnology
fluorescence lifetime imaging
FLIM
time-correlated singlephoton counting
TCSPC

Access to Document

10.1364/OL.40.000336

Li-etal-OL-2015-Fast-bi-exponential-fluorescence-lifetime-imaging-analysis
Gold Open Access
Final published version, 571 KB

Cite this

@article{0457a7d3a8634500a6df850981f3fcf5,

title = "Fast bi-exponential fluorescence lifetime imaging analysis methods",

abstract = "A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers. ",

keywords = "coherence and statistical optics, photon counting, medical optics and biotechnology, fluorescence lifetime imaging, FLIM, time-correlated singlephoton counting, TCSPC",

author = "Li, {David Day-Uei} and Hongqi Yu and Yu Chen",

note = " {\textcopyright} 2014 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.",

year = "2015",

month = feb,

day = "1",

doi = "10.1364/OL.40.000336",

language = "English",

volume = "40",

pages = "336--339",

journal = "Optics Letters",

issn = "0146-9592",

publisher = "Optical Society of America",

number = "3",

}

TY - JOUR

T1 - Fast bi-exponential fluorescence lifetime imaging analysis methods

AU - Li, David Day-Uei

AU - Yu, Hongqi

AU - Chen, Yu

N1 - © 2014 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.

PY - 2015/2/1

Y1 - 2015/2/1

N2 - A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.

AB - A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.

KW - coherence and statistical optics

KW - photon counting

KW - medical optics and biotechnology

KW - fluorescence lifetime imaging

KW - FLIM

KW - time-correlated singlephoton counting

KW - TCSPC

UR - http://www.opticsinfobase.org/ol

U2 - 10.1364/OL.40.000336

DO - 10.1364/OL.40.000336

M3 - Article

SN - 0146-9592

VL - 40

SP - 336

EP - 339

JO - Optics Letters

JF - Optics Letters

IS - 3

ER -

Fast bi-exponential fluorescence lifetime imaging analysis methods

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Global Engagements: David Li National University of Defence Technology

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Cite this

Fast bi-exponential fluorescence lifetime imaging analysis methods

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

Global Engagements: David Li National University of Defence Technology

Research output

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Cite this