Fast bi-exponential fluorescence lifetime imaging analysis methods

Research output: Contribution to journalArticle

9 Citations (Scopus)
52 Downloads (Pure)


A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are non-iterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They can be applicable to single-channel or 2D multi-channel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed non-fitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.
Original languageEnglish
Pages (from-to)336-339
Number of pages4
JournalOptics Letters
Issue number3
Early online date21 Jan 2015
Publication statusPublished - 1 Feb 2015


  • coherence and statistical optics
  • photon counting
  • medical optics and biotechnology
  • fluorescence lifetime imaging
  • FLIM
  • time-correlated singlephoton counting


Global Engagements: David Li National University of Defence Technology

Li, D.


Project: Internally funded project

Research Output

  • 9 Citations
  • 1 Article

Revealing the photophysics of gold-nanobeacons via time-resolved fluorescence spectroscopy

Wei, G., Simionesie, D., Sefcik, J., Sutter, J. U., Xue, Q., Yu, J., Wang, J., Birch, D. J. S. & Chen, Y., 9 Dec 2015, In : Optics Letters. 40, 24, p. 5738–5741 4 p.

Research output: Contribution to journalArticle

Open Access
3 Citations (Scopus)
48 Downloads (Pure)

Cite this