Facile DNA immobilization on surfaces through a catecholamine polymer

H. O. Ham, Z. Q. Liu, K. H. A. Lau, H. Lee, P. B. Messersmith

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Simple immersion of noble metals, oxides, semiconductors, and synthetic polymer substrates in a mussel-mimetic catecholamine polymer solution p(DOMA-AEMA) leads to formation of a thin film on the substrate. The resulting coated substrates can bind DNA without further surface treatment. This approach provides a new entrance to DNA microarray fabrication.
Original languageEnglish
Pages (from-to)732-736
Number of pages5
JournalAngewandte Chemie International Edition
Volume50
Issue number3
Early online date22 Dec 2010
DOIs
Publication statusPublished - 17 Jan 2011

Fingerprint

Immobilization
Catecholamines
Polymers
Semiconductors
Bivalvia
DNA
Immersion
Substrates
Oligonucleotide Array Sequence Analysis
Oxides
Metals
Polymer solutions
Microarrays
Precious metals
Surface treatment
Fabrication
Thin films
Oxide semiconductors

Keywords

  • fluorescence fabrication strategies
  • biomimetic synthesis
  • biosensors
  • catecholamine DNA
  • immobilization
  • surface modification
  • covalent attachment
  • microarray technology
  • mytilus-edulis
  • gold surfaces
  • glass slides
  • tof-sims
  • oligonucleotides

Cite this

Ham, H. O. ; Liu, Z. Q. ; Lau, K. H. A. ; Lee, H. ; Messersmith, P. B. / Facile DNA immobilization on surfaces through a catecholamine polymer. In: Angewandte Chemie International Edition. 2011 ; Vol. 50, No. 3. pp. 732-736.
@article{76e3938bc9e147d5bfb14898ee2c2292,
title = "Facile DNA immobilization on surfaces through a catecholamine polymer",
abstract = "Simple immersion of noble metals, oxides, semiconductors, and synthetic polymer substrates in a mussel-mimetic catecholamine polymer solution p(DOMA-AEMA) leads to formation of a thin film on the substrate. The resulting coated substrates can bind DNA without further surface treatment. This approach provides a new entrance to DNA microarray fabrication.",
keywords = "fluorescence fabrication strategies, biomimetic synthesis, biosensors, catecholamine DNA, immobilization, surface modification, covalent attachment, microarray technology, mytilus-edulis, gold surfaces, glass slides, tof-sims, oligonucleotides",
author = "Ham, {H. O.} and Liu, {Z. Q.} and Lau, {K. H. A.} and H. Lee and Messersmith, {P. B.}",
note = "M1 - 3 Times Cited: 49",
year = "2011",
month = "1",
day = "17",
doi = "10.1002/anie.201005001",
language = "English",
volume = "50",
pages = "732--736",
journal = "Angewandte Chemie International Edition",
issn = "1433-7851",
number = "3",

}

Facile DNA immobilization on surfaces through a catecholamine polymer. / Ham, H. O.; Liu, Z. Q.; Lau, K. H. A.; Lee, H.; Messersmith, P. B.

In: Angewandte Chemie International Edition, Vol. 50, No. 3, 17.01.2011, p. 732-736.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Facile DNA immobilization on surfaces through a catecholamine polymer

AU - Ham, H. O.

AU - Liu, Z. Q.

AU - Lau, K. H. A.

AU - Lee, H.

AU - Messersmith, P. B.

N1 - M1 - 3 Times Cited: 49

PY - 2011/1/17

Y1 - 2011/1/17

N2 - Simple immersion of noble metals, oxides, semiconductors, and synthetic polymer substrates in a mussel-mimetic catecholamine polymer solution p(DOMA-AEMA) leads to formation of a thin film on the substrate. The resulting coated substrates can bind DNA without further surface treatment. This approach provides a new entrance to DNA microarray fabrication.

AB - Simple immersion of noble metals, oxides, semiconductors, and synthetic polymer substrates in a mussel-mimetic catecholamine polymer solution p(DOMA-AEMA) leads to formation of a thin film on the substrate. The resulting coated substrates can bind DNA without further surface treatment. This approach provides a new entrance to DNA microarray fabrication.

KW - fluorescence fabrication strategies

KW - biomimetic synthesis

KW - biosensors

KW - catecholamine DNA

KW - immobilization

KW - surface modification

KW - covalent attachment

KW - microarray technology

KW - mytilus-edulis

KW - gold surfaces

KW - glass slides

KW - tof-sims

KW - oligonucleotides

UR - http://onlinelibrary.wiley.com/journal/10.1002/%28ISSN%291521-3773

U2 - 10.1002/anie.201005001

DO - 10.1002/anie.201005001

M3 - Article

VL - 50

SP - 732

EP - 736

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

SN - 1433-7851

IS - 3

ER -