Fabricating protein immunoassay arrays on nitrocellulose using Dip-pen lithography techniques

Eleanore Jane Irvine, Aaron Hernandez-Santana, Karen Faulds, Duncan Graham

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Advancements in lithography methods for printing biomolecules on surfaces are proving to be potentially beneficial for disease screening and biological research. Dip-pen nanolithography (DPN) is a versatile micro and nanofabrication technique that has the ability to produce functional biomolecule arrays. The greatest advantage, with respect to the printing mechanism, is that DPN adheres to the sensitive mild conditions required for biomolecules such as proteins. We have developed an optimised, high-throughput printing technique for fabricating protein arrays using DPN. This study highlights the fabrication of a prostate specific antigen (PSA) immunoassay detectable by fluorescence. Spot sizes are typically no larger than 8 mm in diameter and limits of detection for PSA are comparable with a commercially available ELISA kit. Furthermore, atomic force microscopy (AFM) analysis of the array surface gives great insight into how the nitrocellulose substrate functions to retain protein integrity. This is the first report of protein arrays being printed on nitrocellulose using the DPN technique and the smallest feature size yet to be achieved on this type of surface. This method offers a significant advance in the ability to produce dense protein arrays on nitrocellulose which are suitable for disease screening using standard fluorescence detection.

LanguageEnglish
Pages2925-2930
Number of pages6
JournalAnalyst
Volume136
Issue number14
DOIs
Publication statusPublished - 2011

Fingerprint

Nitrocellulose
Protein Array Analysis
Printing
Collodion
immunoassay
Nanolithography
Immunoassay
Lithography
dip
Prostate-Specific Antigen
Proteins
Biomolecules
protein
Fluorescence
Antigens
antigen
Atomic Force Microscopy
Screening
fluorescence
Limit of Detection

Keywords

  • antibody microarrays
  • nanolithography
  • nanoarrays
  • oligonucleotides
  • nanostructures
  • assay
  • TIC - Bionanotechnology

Cite this

Irvine, Eleanore Jane ; Hernandez-Santana, Aaron ; Faulds, Karen ; Graham, Duncan. / Fabricating protein immunoassay arrays on nitrocellulose using Dip-pen lithography techniques. In: Analyst. 2011 ; Vol. 136, No. 14. pp. 2925-2930.
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Fabricating protein immunoassay arrays on nitrocellulose using Dip-pen lithography techniques. / Irvine, Eleanore Jane; Hernandez-Santana, Aaron; Faulds, Karen; Graham, Duncan.

In: Analyst, Vol. 136, No. 14, 2011, p. 2925-2930.

Research output: Contribution to journalArticle

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