Abstract
Advancements in lithography methods for printing biomolecules on surfaces are proving to be potentially beneficial for disease screening and biological research. Dip-pen nanolithography (DPN) is a versatile micro and nanofabrication technique that has the ability to produce functional biomolecule arrays. The greatest advantage, with respect to the printing mechanism, is that DPN adheres to the sensitive mild conditions required for biomolecules such as proteins. We have developed an optimised, high-throughput printing technique for fabricating protein arrays using DPN. This study highlights the fabrication of a prostate specific antigen (PSA) immunoassay detectable by fluorescence. Spot sizes are typically no larger than 8 mm in diameter and limits of detection for PSA are comparable with a commercially available ELISA kit. Furthermore, atomic force microscopy (AFM) analysis of the array surface gives great insight into how the nitrocellulose substrate functions to retain protein integrity. This is the first report of protein arrays being printed on nitrocellulose using the DPN technique and the smallest feature size yet to be achieved on this type of surface. This method offers a significant advance in the ability to produce dense protein arrays on nitrocellulose which are suitable for disease screening using standard fluorescence detection.
| Language | English |
|---|---|
| Pages | 2925-2930 |
| Number of pages | 6 |
| Journal | Analyst |
| Volume | 136 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2011 |
Fingerprint
Keywords
- antibody microarrays
- nanolithography
- nanoarrays
- oligonucleotides
- nanostructures
- assay
- TIC - Bionanotechnology
Cite this
}
Fabricating protein immunoassay arrays on nitrocellulose using Dip-pen lithography techniques. / Irvine, Eleanore Jane; Hernandez-Santana, Aaron; Faulds, Karen; Graham, Duncan.
In: Analyst, Vol. 136, No. 14, 2011, p. 2925-2930.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fabricating protein immunoassay arrays on nitrocellulose using Dip-pen lithography techniques
AU - Irvine, Eleanore Jane
AU - Hernandez-Santana, Aaron
AU - Faulds, Karen
AU - Graham, Duncan
PY - 2011
Y1 - 2011
N2 - Advancements in lithography methods for printing biomolecules on surfaces are proving to be potentially beneficial for disease screening and biological research. Dip-pen nanolithography (DPN) is a versatile micro and nanofabrication technique that has the ability to produce functional biomolecule arrays. The greatest advantage, with respect to the printing mechanism, is that DPN adheres to the sensitive mild conditions required for biomolecules such as proteins. We have developed an optimised, high-throughput printing technique for fabricating protein arrays using DPN. This study highlights the fabrication of a prostate specific antigen (PSA) immunoassay detectable by fluorescence. Spot sizes are typically no larger than 8 mm in diameter and limits of detection for PSA are comparable with a commercially available ELISA kit. Furthermore, atomic force microscopy (AFM) analysis of the array surface gives great insight into how the nitrocellulose substrate functions to retain protein integrity. This is the first report of protein arrays being printed on nitrocellulose using the DPN technique and the smallest feature size yet to be achieved on this type of surface. This method offers a significant advance in the ability to produce dense protein arrays on nitrocellulose which are suitable for disease screening using standard fluorescence detection.
AB - Advancements in lithography methods for printing biomolecules on surfaces are proving to be potentially beneficial for disease screening and biological research. Dip-pen nanolithography (DPN) is a versatile micro and nanofabrication technique that has the ability to produce functional biomolecule arrays. The greatest advantage, with respect to the printing mechanism, is that DPN adheres to the sensitive mild conditions required for biomolecules such as proteins. We have developed an optimised, high-throughput printing technique for fabricating protein arrays using DPN. This study highlights the fabrication of a prostate specific antigen (PSA) immunoassay detectable by fluorescence. Spot sizes are typically no larger than 8 mm in diameter and limits of detection for PSA are comparable with a commercially available ELISA kit. Furthermore, atomic force microscopy (AFM) analysis of the array surface gives great insight into how the nitrocellulose substrate functions to retain protein integrity. This is the first report of protein arrays being printed on nitrocellulose using the DPN technique and the smallest feature size yet to be achieved on this type of surface. This method offers a significant advance in the ability to produce dense protein arrays on nitrocellulose which are suitable for disease screening using standard fluorescence detection.
KW - antibody microarrays
KW - nanolithography
KW - nanoarrays
KW - oligonucleotides
KW - nanostructures
KW - assay
KW - TIC - Bionanotechnology
UR - http://www.scopus.com/inward/record.url?scp=79959800990&partnerID=8YFLogxK
U2 - 10.1039/c1an15178a
DO - 10.1039/c1an15178a
M3 - Article
VL - 136
SP - 2925
EP - 2930
JO - Analyst
T2 - Analyst
JF - Analyst
SN - 0003-2654
IS - 14
ER -