Extraction of ergosterol from peaty soils and determination by high performance liquid chromatography

P Anderson, Christine Davidson, David Littlejohn, Allan M. Ure, Charles A. Shand, Martin V. Cheshire

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The ergosterol content of soil can be used as an indicator of fungal activity. A method has been developed for the extraction and determination of ergosterol in organic soils, as part of a study to assess the correlation between fungal activity and the sequestration of metal pollutants. The moisture content of the soil affected the extraction process. Four consecutive extractions with methanol removed >95% of the ergosterol that can be obtained from the fresh sample (63% moisture) by exhaustive extraction. By freeze drying the soils prior to extraction (a) up to 35% more ergosterol was extracted after a single extraction, (b) >90% of the recoverable ergosterol was collected in two extractions and (c) the repeatability of the extraction was improved. Storage of soil extracts in the absence of light prevents degradation of ergosterol. A previously reported method for determination of ergosterol by HPLC has been improved by modification of the eluant composition. With 46% methanol/46% acetonitrile/8% dichloromethane, ergosterol was eluted with good resolution approximately 8 min after injection of 20 mul of the extract. The detection limit of the HPLC method was 0.5 mug/ml ergosterol, equivalent to 0.06 mug/g in 25 g fresh soil. Changes in ergosterol contents of peaty soil treated with fungicide, and in samples of the peaty podzol and a humus iron podzol in the vicinity of fungal fruiting bodies, have been determined.

Original languageEnglish
Pages (from-to)711-720
Number of pages10
JournalTalanta
Volume41
Issue number5
DOIs
Publication statusPublished - May 1994

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Ergosterol
High performance liquid chromatography
Soil
High Pressure Liquid Chromatography
Soils
Methanol
Fungal Fruiting Bodies
Moisture
Fungicides
Freeze Drying
Methylene Chloride
Limit of Detection
Drying
Iron
Metals

Keywords

  • fungicide
  • ergosterol
  • metal pollutants
  • fungal activity
  • soil

Cite this

Anderson, P ; Davidson, Christine ; Littlejohn, David ; Ure, Allan M. ; Shand, Charles A. ; Cheshire, Martin V. / Extraction of ergosterol from peaty soils and determination by high performance liquid chromatography. In: Talanta. 1994 ; Vol. 41, No. 5. pp. 711-720.
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Extraction of ergosterol from peaty soils and determination by high performance liquid chromatography. / Anderson, P ; Davidson, Christine; Littlejohn, David; Ure, Allan M.; Shand, Charles A.; Cheshire, Martin V.

In: Talanta, Vol. 41, No. 5, 05.1994, p. 711-720.

Research output: Contribution to journalArticle

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