Extensional flow of hyaluronic acid solutions in an optimized micofluidic cross-slot device

S.J. Haward, A. Jaishankar, Monica Oliveira, M.A. Alves, G.H. McKinley

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We utilize a recently developed microfluidic device, the Optimized Shape Cross-slot Extensional Rheometer (OSCER), to study the elongational flow behavior and rheological properties of hyaluronic acid (HA) solutions representative of the synovial fluid (SF) found in the knee joint. The OSCER geometry is a stagnation point device that imposes a planar extensional flow with a homogenous extension rate over a significant length of the inlet and outlet channel axes. Due to the compressive nature of the flow generated along the inlet channels, and the planar elongational flow along the outlet channels, the flow field in the OSCER device can also be considered as representative of the flow field that arises between compressing articular cartilage layers of the knee joints during running or jumping movements. Full-field birefringence microscopy measurements demonstrate a high degree of localized macromolecular orientation along streamlines passing close to the stagnation point of the OSCER device, while micro-particle image velocimetry is used to quantify the flow kinematics. The stress-optical rule is used to assess the local extensional viscosity in the elongating fluid elements as a function of the measured deformation rate. The large limiting values of the dimensionless Trouton ratio, Tr ∼ O(50), demonstrate that these fluids are highly extensional-thickening, providing a clear mechanism for the load-dampening properties of SF. The results also indicate the potential for utilizing the OSCER in screening of physiological SF samples, which will lead to improved understanding of, and therapies for, disease progression in arthritis sufferers.
LanguageEnglish
PagesArticle 044108
JournalBiomicrofluidics
Volume7
Issue number4
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Hyaluronic acid
rheometers
Synovial Fluid
Rheometers
Hyaluronic Acid
slots
Lab-On-A-Chip Devices
Knee Joint
Equipment and Supplies
acids
Fluids
fluids
stagnation point
Birefringence
outlets
Rheology
Articular Cartilage
Flow fields
flow distribution
Biomechanical Phenomena

Keywords

  • extensional flow
  • hyaluronic acid
  • microfluidic device

Cite this

Haward, S.J. ; Jaishankar, A. ; Oliveira, Monica ; Alves, M.A. ; McKinley, G.H. / Extensional flow of hyaluronic acid solutions in an optimized micofluidic cross-slot device. In: Biomicrofluidics. 2013 ; Vol. 7, No. 4. pp. Article 044108.
@article{6f6f6d94d95d46cc8bb1b83d170547f4,
title = "Extensional flow of hyaluronic acid solutions in an optimized micofluidic cross-slot device",
abstract = "We utilize a recently developed microfluidic device, the Optimized Shape Cross-slot Extensional Rheometer (OSCER), to study the elongational flow behavior and rheological properties of hyaluronic acid (HA) solutions representative of the synovial fluid (SF) found in the knee joint. The OSCER geometry is a stagnation point device that imposes a planar extensional flow with a homogenous extension rate over a significant length of the inlet and outlet channel axes. Due to the compressive nature of the flow generated along the inlet channels, and the planar elongational flow along the outlet channels, the flow field in the OSCER device can also be considered as representative of the flow field that arises between compressing articular cartilage layers of the knee joints during running or jumping movements. Full-field birefringence microscopy measurements demonstrate a high degree of localized macromolecular orientation along streamlines passing close to the stagnation point of the OSCER device, while micro-particle image velocimetry is used to quantify the flow kinematics. The stress-optical rule is used to assess the local extensional viscosity in the elongating fluid elements as a function of the measured deformation rate. The large limiting values of the dimensionless Trouton ratio, Tr ∼ O(50), demonstrate that these fluids are highly extensional-thickening, providing a clear mechanism for the load-dampening properties of SF. The results also indicate the potential for utilizing the OSCER in screening of physiological SF samples, which will lead to improved understanding of, and therapies for, disease progression in arthritis sufferers.",
keywords = "extensional flow , hyaluronic acid, microfluidic device",
author = "S.J. Haward and A. Jaishankar and Monica Oliveira and M.A. Alves and G.H. McKinley",
year = "2013",
month = "7",
doi = "10.1063/1.4816708",
language = "English",
volume = "7",
pages = "Article 044108",
journal = "Biomicrofluidics",
issn = "1932-1058",
number = "4",

}

Extensional flow of hyaluronic acid solutions in an optimized micofluidic cross-slot device. / Haward, S.J.; Jaishankar, A.; Oliveira, Monica; Alves, M.A.; McKinley, G.H.

In: Biomicrofluidics, Vol. 7, No. 4, 07.2013, p. Article 044108.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Extensional flow of hyaluronic acid solutions in an optimized micofluidic cross-slot device

AU - Haward, S.J.

AU - Jaishankar, A.

AU - Oliveira, Monica

AU - Alves, M.A.

AU - McKinley, G.H.

PY - 2013/7

Y1 - 2013/7

N2 - We utilize a recently developed microfluidic device, the Optimized Shape Cross-slot Extensional Rheometer (OSCER), to study the elongational flow behavior and rheological properties of hyaluronic acid (HA) solutions representative of the synovial fluid (SF) found in the knee joint. The OSCER geometry is a stagnation point device that imposes a planar extensional flow with a homogenous extension rate over a significant length of the inlet and outlet channel axes. Due to the compressive nature of the flow generated along the inlet channels, and the planar elongational flow along the outlet channels, the flow field in the OSCER device can also be considered as representative of the flow field that arises between compressing articular cartilage layers of the knee joints during running or jumping movements. Full-field birefringence microscopy measurements demonstrate a high degree of localized macromolecular orientation along streamlines passing close to the stagnation point of the OSCER device, while micro-particle image velocimetry is used to quantify the flow kinematics. The stress-optical rule is used to assess the local extensional viscosity in the elongating fluid elements as a function of the measured deformation rate. The large limiting values of the dimensionless Trouton ratio, Tr ∼ O(50), demonstrate that these fluids are highly extensional-thickening, providing a clear mechanism for the load-dampening properties of SF. The results also indicate the potential for utilizing the OSCER in screening of physiological SF samples, which will lead to improved understanding of, and therapies for, disease progression in arthritis sufferers.

AB - We utilize a recently developed microfluidic device, the Optimized Shape Cross-slot Extensional Rheometer (OSCER), to study the elongational flow behavior and rheological properties of hyaluronic acid (HA) solutions representative of the synovial fluid (SF) found in the knee joint. The OSCER geometry is a stagnation point device that imposes a planar extensional flow with a homogenous extension rate over a significant length of the inlet and outlet channel axes. Due to the compressive nature of the flow generated along the inlet channels, and the planar elongational flow along the outlet channels, the flow field in the OSCER device can also be considered as representative of the flow field that arises between compressing articular cartilage layers of the knee joints during running or jumping movements. Full-field birefringence microscopy measurements demonstrate a high degree of localized macromolecular orientation along streamlines passing close to the stagnation point of the OSCER device, while micro-particle image velocimetry is used to quantify the flow kinematics. The stress-optical rule is used to assess the local extensional viscosity in the elongating fluid elements as a function of the measured deformation rate. The large limiting values of the dimensionless Trouton ratio, Tr ∼ O(50), demonstrate that these fluids are highly extensional-thickening, providing a clear mechanism for the load-dampening properties of SF. The results also indicate the potential for utilizing the OSCER in screening of physiological SF samples, which will lead to improved understanding of, and therapies for, disease progression in arthritis sufferers.

KW - extensional flow

KW - hyaluronic acid

KW - microfluidic device

UR - http://www.scopus.com/inward/record.url?scp=84883356131&partnerID=8YFLogxK

UR - http://bmf.aip.org/resource/1/biomgb/v7/i4/p044108_s1?isAuthorized=no

U2 - 10.1063/1.4816708

DO - 10.1063/1.4816708

M3 - Article

VL - 7

SP - Article 044108

JO - Biomicrofluidics

T2 - Biomicrofluidics

JF - Biomicrofluidics

SN - 1932-1058

IS - 4

ER -