Expression of the filarial nematode phosphorylcholine-containing glycoprotein, ES62, is stage specific

G Stepek, M Auchie, R Tate, K Watson, D G Russell, E Devaney, W Harnett

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

ES62, an immunomodulatory phosphorylcholine-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae, has previously been shown to be produced by L4 larvae and adult worms only. However, homologous sequences to ES62 have recently been found in L1 and L3 cDNA libraries of certain human filarial nematodes. Therefore, the various stages of A. viteae were re-examined and it was again found that only the post-L3 stages secreted ES62. Synthesis but not secretion by earlier stages was ruled out by examination of the protein content of whole worm extracts and by immunoelectron microscopy. However, examination by PCR of the mRNA for ES62 revealed that it was found in the L1 and L3 larvae. This may explain why homologous sequences to ES62 have been found in Brugia malayi and Onchocerca volvulus larval cDNA libraries. It also suggests that filarial nematodes, in general, may secrete ES62. To obtain evidence for this, we investigated production by Brugia pahangi, a close relation of B. malayi. We found that ES62 was indeed secreted but, as with A. viteae, only by the post-L3 stages, although again the mRNA for ES62 could be detected in the earlier stages. Overall our results suggest that production of ES62 is not species specific, that it is indeed stage specific, and that this may be due to post-transcriptional control of expression.
LanguageEnglish
Pages155-64
Number of pages10
JournalParasitology
Volume125
Issue numberPt 2
DOIs
Publication statusPublished - 2002

Fingerprint

Acanthocheilonema viteae
Brugia malayi
Phosphorylcholine
Sequence Homology
Gene Library
Larva
glycoproteins
Acanthocheilonema
Glycoproteins
Brugia pahangi
Nematoda
Onchocerca volvulus
sequence homology
cDNA libraries
Messenger RNA
Immunoelectron Microscopy
Rodentia
larvae
Polymerase Chain Reaction
microscopy

Keywords

  • amino acid sequence
  • animals
  • brugia pahangi
  • dipetalonema
  • electrophoresis, polyacrylamide gel
  • gene expression regulation
  • gerbillinae
  • Glycoproteins
  • larva
  • Mice
  • Mice, Inbred BALB C
  • molecular sequence data
  • phosphorylcholine
  • sequence homology
  • amino acid

Cite this

Stepek, G ; Auchie, M ; Tate, R ; Watson, K ; Russell, D G ; Devaney, E ; Harnett, W. / Expression of the filarial nematode phosphorylcholine-containing glycoprotein, ES62, is stage specific. In: Parasitology. 2002 ; Vol. 125, No. Pt 2. pp. 155-64.
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Expression of the filarial nematode phosphorylcholine-containing glycoprotein, ES62, is stage specific. / Stepek, G; Auchie, M; Tate, R; Watson, K; Russell, D G; Devaney, E; Harnett, W.

In: Parasitology, Vol. 125, No. Pt 2, 2002, p. 155-64.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of the filarial nematode phosphorylcholine-containing glycoprotein, ES62, is stage specific

AU - Stepek, G

AU - Auchie, M

AU - Tate, R

AU - Watson, K

AU - Russell, D G

AU - Devaney, E

AU - Harnett, W

PY - 2002

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N2 - ES62, an immunomodulatory phosphorylcholine-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae, has previously been shown to be produced by L4 larvae and adult worms only. However, homologous sequences to ES62 have recently been found in L1 and L3 cDNA libraries of certain human filarial nematodes. Therefore, the various stages of A. viteae were re-examined and it was again found that only the post-L3 stages secreted ES62. Synthesis but not secretion by earlier stages was ruled out by examination of the protein content of whole worm extracts and by immunoelectron microscopy. However, examination by PCR of the mRNA for ES62 revealed that it was found in the L1 and L3 larvae. This may explain why homologous sequences to ES62 have been found in Brugia malayi and Onchocerca volvulus larval cDNA libraries. It also suggests that filarial nematodes, in general, may secrete ES62. To obtain evidence for this, we investigated production by Brugia pahangi, a close relation of B. malayi. We found that ES62 was indeed secreted but, as with A. viteae, only by the post-L3 stages, although again the mRNA for ES62 could be detected in the earlier stages. Overall our results suggest that production of ES62 is not species specific, that it is indeed stage specific, and that this may be due to post-transcriptional control of expression.

AB - ES62, an immunomodulatory phosphorylcholine-containing glycoprotein secreted by the rodent filarial nematode Acanthocheilonema viteae, has previously been shown to be produced by L4 larvae and adult worms only. However, homologous sequences to ES62 have recently been found in L1 and L3 cDNA libraries of certain human filarial nematodes. Therefore, the various stages of A. viteae were re-examined and it was again found that only the post-L3 stages secreted ES62. Synthesis but not secretion by earlier stages was ruled out by examination of the protein content of whole worm extracts and by immunoelectron microscopy. However, examination by PCR of the mRNA for ES62 revealed that it was found in the L1 and L3 larvae. This may explain why homologous sequences to ES62 have been found in Brugia malayi and Onchocerca volvulus larval cDNA libraries. It also suggests that filarial nematodes, in general, may secrete ES62. To obtain evidence for this, we investigated production by Brugia pahangi, a close relation of B. malayi. We found that ES62 was indeed secreted but, as with A. viteae, only by the post-L3 stages, although again the mRNA for ES62 could be detected in the earlier stages. Overall our results suggest that production of ES62 is not species specific, that it is indeed stage specific, and that this may be due to post-transcriptional control of expression.

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KW - dipetalonema

KW - electrophoresis, polyacrylamide gel

KW - gene expression regulation

KW - gerbillinae

KW - Glycoproteins

KW - larva

KW - Mice

KW - Mice, Inbred BALB C

KW - molecular sequence data

KW - phosphorylcholine

KW - sequence homology

KW - amino acid

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EP - 164

JO - Parasitology

T2 - Parasitology

JF - Parasitology

SN - 0031-1820

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ER -