Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition

M. Kataropoulou; C.J. Henderson; M.H. Grant

doi:10.1016/S0300-483X(03)00254-3

Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition

M. Kataropoulou, C.J. Henderson, M.H. Grant

Research output: Contribution to journal › Article › peer-review

Abstract

The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v), and crosslinking the gels with either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression and total activity of glutathione-S-transferases (GSTs) in hepatocytes cultured for 7 days. GSTs are a family of isoenzymes. The main isoenzyme in the male rat liver is the alpha form (which exists as several subunits (Ya and Yc2 were measured here)). The pi form (subunit Yf) is not normally present in liver, but is expressed in conditions where the liver cells are de-differentiated.

Original language	English
Pages (from-to)	87-88
Number of pages	1
Journal	Toxicology
Volume	192
DOIs	https://doi.org/10.1016/S0300-483X(03)00254-3
Publication status	Published - 2002

Keywords

liver
toxicology
bioengineering
collagen
hepatocytes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0300-483X(03)00254-3

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@article{928bd5b8c91e4255a6edf4b059acffe1,

title = "Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition",

abstract = "The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v), and crosslinking the gels with either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression and total activity of glutathione-S-transferases (GSTs) in hepatocytes cultured for 7 days. GSTs are a family of isoenzymes. The main isoenzyme in the male rat liver is the alpha form (which exists as several subunits (Ya and Yc2 were measured here)). The pi form (subunit Yf) is not normally present in liver, but is expressed in conditions where the liver cells are de-differentiated.",

keywords = "liver, toxicology, bioengineering, collagen, hepatocytes",

author = "M. Kataropoulou and C.J. Henderson and M.H. Grant",

note = "Selected Papers and Abstracts of the British Toxicology Society Annual Congress",

year = "2002",

doi = "10.1016/S0300-483X(03)00254-3",

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volume = "192",

pages = "87--88",

journal = "Toxicology",

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TY - JOUR

T1 - Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition

AU - Kataropoulou, M.

AU - Henderson, C.J.

AU - Grant, M.H.

N1 - Selected Papers and Abstracts of the British Toxicology Society Annual Congress

PY - 2002

Y1 - 2002

N2 - The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v), and crosslinking the gels with either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression and total activity of glutathione-S-transferases (GSTs) in hepatocytes cultured for 7 days. GSTs are a family of isoenzymes. The main isoenzyme in the male rat liver is the alpha form (which exists as several subunits (Ya and Yc2 were measured here)). The pi form (subunit Yf) is not normally present in liver, but is expressed in conditions where the liver cells are de-differentiated.

AB - The use of primary hepatocyte cultures as in vitro models for studying xenobiotic metabolism and toxicity is limited by the loss of liver-specific differentiated functions with time in culture. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen gels (0.3% w/v), and crosslinking the gels with either 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) or 1,6-diaminohexane (DAH) on the expression and total activity of glutathione-S-transferases (GSTs) in hepatocytes cultured for 7 days. GSTs are a family of isoenzymes. The main isoenzyme in the male rat liver is the alpha form (which exists as several subunits (Ya and Yc2 were measured here)). The pi form (subunit Yf) is not normally present in liver, but is expressed in conditions where the liver cells are de-differentiated.

KW - liver

KW - toxicology

KW - bioengineering

KW - collagen

KW - hepatocytes

UR - http://dx.doi.org/10.1016/S0300-483X(03)00254-3

U2 - 10.1016/S0300-483X(03)00254-3

DO - 10.1016/S0300-483X(03)00254-3

M3 - Article

SN - 0300-483X

VL - 192

SP - 87

EP - 88

JO - Toxicology

JF - Toxicology

ER -

Expression of glutathione-S-transferases in hepatocytes cultured on collagen gels of different composition

Abstract

Keywords

UN SDGs

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