Abstract
We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
Original language | English |
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Article number | E20 |
Number of pages | 5 |
Journal | Nucleic Acids Research |
Volume | 34 |
Early online date | 2 Sep 2006 |
DOIs | |
Publication status | Published - 2006 |
Keywords
- genetics
- streptomyces