Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces

G. Khodakaramian; B. Gust; S. Lissenden; L. Moir; P. Hoskisson; K.F. Chater; M.C.M. Smith

doi:10.1093/nar/gnj019

Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces

G. Khodakaramian, B. Gust, S. Lissenden, L. Moir, P. Hoskisson, K.F. Chater, M.C.M. Smith

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.

Original language	English
Article number	E20
Number of pages	5
Journal	Nucleic Acids Research
Volume	34
Early online date	2 Sep 2006
DOIs	https://doi.org/10.1093/nar/gnj019
Publication status	Published - 2006

Keywords

genetics
streptomyces

Access to Document

10.1093/nar/gnj019

http://dx.doi.org/10.1093/nar/gnj019

Cite this

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title = "Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces",

abstract = "We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.",

keywords = "genetics, streptomyces",

author = "G. Khodakaramian and B. Gust and S. Lissenden and L. Moir and P. Hoskisson and K.F. Chater and M.C.M. Smith",

year = "2006",

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language = "English",

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TY - JOUR

T1 - Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces

AU - Khodakaramian, G.

AU - Gust, B.

AU - Lissenden, S.

AU - Moir, L.

AU - Hoskisson, P.

AU - Chater, K.F.

AU - Smith, M.C.M.

PY - 2006

Y1 - 2006

N2 - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.

AB - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.

KW - genetics

KW - streptomyces

UR - http://dx.doi.org/10.1093/nar/gnj019

U2 - 10.1093/nar/gnj019

DO - 10.1093/nar/gnj019

M3 - Article

VL - 34

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

M1 - E20

ER -