Abstract
We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
| Original language | English |
|---|---|
| Article number | E20 |
| Number of pages | 5 |
| Journal | Nucleic Acids Research |
| Volume | 34 |
| Early online date | 2 Sep 2006 |
| DOIs | |
| Publication status | Published - 2006 |
Keywords
- genetics
- streptomyces
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Khodakaramian, G., Gust, B., Lissenden, S., Moir, L., Hoskisson, P., Chater, K. F., & Smith, M. C. M. (2006). Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces. Nucleic Acids Research, 34, [E20]. https://doi.org/10.1093/nar/gnj019