Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces

G. Khodakaramian, B. Gust, S. Lissenden, L. Moir, P. Hoskisson, K.F. Chater, M.C.M. Smith

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
LanguageEnglish
Article numberE20
Number of pages5
JournalNucleic Acids Research
Volume34
Early online date2 Sep 2006
DOIs
Publication statusPublished - 2006

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Streptomyces
Bacteriophages
Infection
Streptomyces coelicolor
Gene Deletion
Cre recombinase
Growth

Keywords

  • genetics
  • streptomyces

Cite this

Khodakaramian, G. ; Gust, B. ; Lissenden, S. ; Moir, L. ; Hoskisson, P. ; Chater, K.F. ; Smith, M.C.M. / Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces. In: Nucleic Acids Research. 2006 ; Vol. 34.
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Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces. / Khodakaramian, G.; Gust, B.; Lissenden, S.; Moir, L.; Hoskisson, P.; Chater, K.F.; Smith, M.C.M.

In: Nucleic Acids Research, Vol. 34, E20, 2006.

Research output: Contribution to journalArticle

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AU - Khodakaramian, G.

AU - Gust, B.

AU - Lissenden, S.

AU - Moir, L.

AU - Hoskisson, P.

AU - Chater, K.F.

AU - Smith, M.C.M.

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AB - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.

KW - genetics

KW - streptomyces

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U2 - 10.1093/nar/gnj019

DO - 10.1093/nar/gnj019

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JO - Nucleic Acids Research

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