Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces

G. Khodakaramian, B. Gust, S. Lissenden, L. Moir, P. Hoskisson, K.F. Chater, M.C.M. Smith

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28 Citations (Scopus)


We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
Original languageEnglish
Article numberE20
Number of pages5
JournalNucleic Acids Research
Early online date2 Sep 2006
Publication statusPublished - 2006


  • genetics
  • streptomyces

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