Abstract
| Language | English |
|---|---|
| Article number | E20 |
| Number of pages | 5 |
| Journal | Nucleic Acids Research |
| Volume | 34 |
| Early online date | 2 Sep 2006 |
| DOIs | |
| Publication status | Published - 2006 |
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Keywords
- genetics
- streptomyces
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Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces. / Khodakaramian, G.; Gust, B.; Lissenden, S.; Moir, L.; Hoskisson, P.; Chater, K.F.; Smith, M.C.M.
In: Nucleic Acids Research, Vol. 34, E20, 2006.Research output: Contribution to journal › Article
TY - JOUR
T1 - Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces
AU - Khodakaramian, G.
AU - Gust, B.
AU - Lissenden, S.
AU - Moir, L.
AU - Hoskisson, P.
AU - Chater, K.F.
AU - Smith, M.C.M.
PY - 2006
Y1 - 2006
N2 - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
AB - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
KW - genetics
KW - streptomyces
UR - http://dx.doi.org/10.1093/nar/gnj019
U2 - 10.1093/nar/gnj019
DO - 10.1093/nar/gnj019
M3 - Article
VL - 34
JO - Nucleic Acids Research
T2 - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
M1 - E20
ER -