Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces

G. Khodakaramian, B. Gust, S. Lissenden, L. Moir, P. Hoskisson, K.F. Chater, M.C.M. Smith

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.
Original languageEnglish
Article numberE20
Number of pages5
JournalNucleic Acids Research
Volume34
Early online date2 Sep 2006
DOIs
Publication statusPublished - 2006

Keywords

  • genetics
  • streptomyces

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