Abstract
| Language | English |
|---|---|
| Pages | 57-72 |
| Number of pages | 16 |
| Journal | Journal of Industrial Microbiology & Biotechnology |
| Volume | 42 |
| Issue number | 1 |
| Early online date | 5 Nov 2014 |
| DOIs | |
| Publication status | Published - 31 Jan 2015 |
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Keywords
- actinobacteria
- biological activity
- Canada
- chemical analysis
- sampling
- microbiology
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Exploring the diversity and metabolic potential of actinomycetes from temperate marine sediments from Newfoundland, Canada. / Duncan, K. R.; Haltli, B.; Gill, K. A.; Correa, H.; Berrué, F.; Kerr, R. G.
In: Journal of Industrial Microbiology & Biotechnology, Vol. 42, No. 1, 31.01.2015, p. 57-72.Research output: Contribution to journal › Article
TY - JOUR
T1 - Exploring the diversity and metabolic potential of actinomycetes from temperate marine sediments from Newfoundland, Canada.
AU - Duncan, K. R.
AU - Haltli, B.
AU - Gill, K. A.
AU - Correa, H.
AU - Berrué, F.
AU - Kerr, R. G.
PY - 2015/1/31
Y1 - 2015/1/31
N2 - Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H'-2.45 to 3.76), although the taxonomic diversity of biotechnologically important actinomycetes could not be fully elucidated due to limited sampling depth. Assessment of culturable actinomycete diversity resulted in the isolation of 360 actinomycetes representing 59 operational taxonomic units, the majority of which (94 %) were Streptomyces. The biotechnological potential of actinomycetes from NL sediments was assessed by bioactivity and metabolomics-based screening of 32 representative isolates. Bioactivity was exhibited by 41 % of isolates, while 11 % exhibited unique chemical signatures in metabolomics screening. Chemical analysis of two isolates resulted in the isolation of the cytotoxic metabolite 1-isopentadecanoyl-3β-D-glucopyranosyl-X-glycerol from Actinoalloteichus sp. 2L868 and sungsanpin from Streptomyces sp. 8LB7. These results demonstrate the potential for the discovery of novel bioactive metabolites from actinomycetes isolated from Atlantic Canadian marine sediments.
AB - Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H'-2.45 to 3.76), although the taxonomic diversity of biotechnologically important actinomycetes could not be fully elucidated due to limited sampling depth. Assessment of culturable actinomycete diversity resulted in the isolation of 360 actinomycetes representing 59 operational taxonomic units, the majority of which (94 %) were Streptomyces. The biotechnological potential of actinomycetes from NL sediments was assessed by bioactivity and metabolomics-based screening of 32 representative isolates. Bioactivity was exhibited by 41 % of isolates, while 11 % exhibited unique chemical signatures in metabolomics screening. Chemical analysis of two isolates resulted in the isolation of the cytotoxic metabolite 1-isopentadecanoyl-3β-D-glucopyranosyl-X-glycerol from Actinoalloteichus sp. 2L868 and sungsanpin from Streptomyces sp. 8LB7. These results demonstrate the potential for the discovery of novel bioactive metabolites from actinomycetes isolated from Atlantic Canadian marine sediments.
KW - actinobacteria
KW - biological activity
KW - Canada
KW - chemical analysis
KW - sampling
KW - microbiology
UR - http://link.springer.com/journal/10295
U2 - 10.1007/s10295-014-1529-x
DO - 10.1007/s10295-014-1529-x
M3 - Article
VL - 42
SP - 57
EP - 72
JO - Journal of Industrial Microbiology & Biotechnology
T2 - Journal of Industrial Microbiology & Biotechnology
JF - Journal of Industrial Microbiology & Biotechnology
SN - 1367-5435
IS - 1
ER -