Exploring the diversity and metabolic potential of actinomycetes from temperate marine sediments from Newfoundland, Canada.

K. R. Duncan, B. Haltli, K. A. Gill, H. Correa, F. Berrué, R. G. Kerr

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H'-2.45 to 3.76), although the taxonomic diversity of biotechnologically important actinomycetes could not be fully elucidated due to limited sampling depth. Assessment of culturable actinomycete diversity resulted in the isolation of 360 actinomycetes representing 59 operational taxonomic units, the majority of which (94 %) were Streptomyces. The biotechnological potential of actinomycetes from NL sediments was assessed by bioactivity and metabolomics-based screening of 32 representative isolates. Bioactivity was exhibited by 41 % of isolates, while 11 % exhibited unique chemical signatures in metabolomics screening. Chemical analysis of two isolates resulted in the isolation of the cytotoxic metabolite 1-isopentadecanoyl-3β-D-glucopyranosyl-X-glycerol from Actinoalloteichus sp. 2L868 and sungsanpin from Streptomyces sp. 8LB7. These results demonstrate the potential for the discovery of novel bioactive metabolites from actinomycetes isolated from Atlantic Canadian marine sediments.
LanguageEnglish
Pages57-72
Number of pages16
JournalJournal of Industrial Microbiology & Biotechnology
Volume42
Issue number1
Early online date5 Nov 2014
DOIs
Publication statusPublished - 31 Jan 2015

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Geologic Sediments
Newfoundland and Labrador
Actinobacteria
Canada
Metabolomics
Streptomyces
Glycerol

Keywords

  • actinobacteria
  • biological activity
  • Canada
  • chemical analysis
  • sampling
  • microbiology

Cite this

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abstract = "Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H'-2.45 to 3.76), although the taxonomic diversity of biotechnologically important actinomycetes could not be fully elucidated due to limited sampling depth. Assessment of culturable actinomycete diversity resulted in the isolation of 360 actinomycetes representing 59 operational taxonomic units, the majority of which (94 {\%}) were Streptomyces. The biotechnological potential of actinomycetes from NL sediments was assessed by bioactivity and metabolomics-based screening of 32 representative isolates. Bioactivity was exhibited by 41 {\%} of isolates, while 11 {\%} exhibited unique chemical signatures in metabolomics screening. Chemical analysis of two isolates resulted in the isolation of the cytotoxic metabolite 1-isopentadecanoyl-3β-D-glucopyranosyl-X-glycerol from Actinoalloteichus sp. 2L868 and sungsanpin from Streptomyces sp. 8LB7. These results demonstrate the potential for the discovery of novel bioactive metabolites from actinomycetes isolated from Atlantic Canadian marine sediments.",
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Exploring the diversity and metabolic potential of actinomycetes from temperate marine sediments from Newfoundland, Canada. / Duncan, K. R.; Haltli, B.; Gill, K. A.; Correa, H.; Berrué, F.; Kerr, R. G.

In: Journal of Industrial Microbiology & Biotechnology, Vol. 42, No. 1, 31.01.2015, p. 57-72.

Research output: Contribution to journalArticle

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