Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy

Amanda Wright, David Burns, B.A. Patterson, S. Poland, G.J. Valentine, J. Girkin

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

We report on the introduction of active optical elements into confocal and multiphoton microscopes in order to reduce the sample-induced aberration. Using a flexible membrane mirror as the active element, the beam entering the rear of the microscope objective is altered to produce the smallest point spread function once it is brought to a focus inside the sample. The conventional approach to adaptive optics, commonly used in astronomy, is to utilise a wavefront sensor to determine the required mirror shape. We have developed a technique that uses optimisation algorithms to improve the returned signal without the use of a wavefront sensor. We have investigated a number of possible optimisation methods, covering hill climbing, genetic algorithms, and more random search methods. The system has demonstrated a significant enhancement in the axial resolution of a confocal microscope when imaging at depth within a sample. We discuss the trade-offs of the various approaches adopted, comparing speed with resolution enhancement.
LanguageEnglish
Pages36-44
Number of pages8
JournalMicroscopy Research and Technique
Volume67
Issue number1
DOIs
Publication statusPublished - 15 Jul 2005

Fingerprint

Adaptive optics
Wavefronts
adaptive optics
Confocal Microscopy
Microscopic examination
Microscopes
Astronomy
microscopy
optimization
Optical transfer function
Optical devices
Aberrations
microscopes
Mirrors
augmentation
Genetic algorithms
point spread functions
astronomy
Imaging techniques
genetic algorithms

Keywords

  • confocal microscopy
  • multiphoton microscopy
  • adaptive optics

Cite this

Wright, Amanda ; Burns, David ; Patterson, B.A. ; Poland, S. ; Valentine, G.J. ; Girkin, J. / Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy. In: Microscopy Research and Technique. 2005 ; Vol. 67, No. 1. pp. 36-44.
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Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy. / Wright, Amanda; Burns, David; Patterson, B.A.; Poland, S.; Valentine, G.J.; Girkin, J.

In: Microscopy Research and Technique, Vol. 67, No. 1, 15.07.2005, p. 36-44.

Research output: Contribution to journalArticle

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