TY - JOUR
T1 - Experimental and computational examination of anastellin (FnIII1c)-polymer interactions
AU - Mallinson, David
AU - Cheung, David L.
AU - Simionesie, Dorin
AU - Mullen, Alexander B.
AU - Zhang, Zhenyu
AU - Lamprou, Dimitrios A.
N1 - This is the peer reviewed version of the following article: [Mallinson, D., Cheung, D. L., Simionesie, D., Mullen, A. B., Zhang, Z., & Lamprou, D. A. (2016). Experimental and computational examination of anastellin (FnIII1c)-polymer interactions. Journal of Biomedical Materials Research Part A.], which has been published in final form at http://dx.doi.org/10.1002/jbm.a.35949. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
PY - 2016/10/24
Y1 - 2016/10/24
N2 - Using a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. The molecular interaction was directly quantified by atomic force microscope (AFM) based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). It was found that the anastellin molecules facilitates a stronger adhesion on polyurethane films (72.0 pN nm-1) than on poly (methyl methacrylate) films (68.6 pN nm-1). This is consistent with the adsorption measurements of anastellin on the two polymeric surfaces, observed by QCM. Molecular dynamics simulations of the behaviour of anastellin on polyurethane in water solution were performed to rationalise the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water.
AB - Using a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. The molecular interaction was directly quantified by atomic force microscope (AFM) based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). It was found that the anastellin molecules facilitates a stronger adhesion on polyurethane films (72.0 pN nm-1) than on poly (methyl methacrylate) films (68.6 pN nm-1). This is consistent with the adsorption measurements of anastellin on the two polymeric surfaces, observed by QCM. Molecular dynamics simulations of the behaviour of anastellin on polyurethane in water solution were performed to rationalise the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water.
KW - atomic force microscopy
KW - molecular dynamics
KW - fibronectin
KW - poly (methyl methacrylate)
KW - polyurethane
UR - http://onlinelibrary.wiley.com/wol1/doi/10.1002/jbm.a.35949/abstract
U2 - 10.1002/jbm.a.35949
DO - 10.1002/jbm.a.35949
M3 - Article
JO - Journal of Biomedical Materials Research Part A
JF - Journal of Biomedical Materials Research Part A
SN - 1549-3296
ER -