Abstract
| Language | English |
|---|---|
| Pages | 628–636 |
| Number of pages | 9 |
| Journal | Experimental Brain Research |
| Volume | 82 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 30 Nov 1990 |
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Keywords
- dopamine
- drinking
- eating
- excitotoxic lesion
- lateral hypothalamus
- N-methyl-d-aspartate
- rats
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Excitotoxic lesions of the lateral hypothalamus made by N-methyl-d-aspartate in the rat : behavioural, histological and biochemical analyses. / Winn, P.; Clark, A.; Hastings, M.; Clark, J.; Latimer, M.; Rugg, E.; Brownlee, B.
In: Experimental Brain Research, Vol. 82, No. 3, 30.11.1990, p. 628–636.Research output: Contribution to journal › Article
TY - JOUR
T1 - Excitotoxic lesions of the lateral hypothalamus made by N-methyl-d-aspartate in the rat
T2 - Experimental Brain Research
AU - Winn, P.
AU - Clark, A.
AU - Hastings, M.
AU - Clark, J.
AU - Latimer, M.
AU - Rugg, E.
AU - Brownlee, B.
PY - 1990/11/30
Y1 - 1990/11/30
N2 - The purpose of this study was to determine whether the excitotoxin N-methyl-d-aspartate (NMDA) could be used to make lesions within the lateral hypothalamus and what effect they had on regulatory behaviour. Larger doses of NMDA were effetive in the lateral hypothalmus but tended to spread into adjacent structures; smaller doses made lesions which were contained within the lateral hypothalamus and zona incerta. Lesions which damaged the lateral hypothalamus and surrounding tissue had no effect on the concentration of dopamine (or its metabolites) in the dorsal or ventral striatum. The large lesions, including extrahypothalamic damage, were associated with long-term deficits in lab chow and water intake, but rats with lesions restricted to the lateral hypothalamus made good recoveries, eating and drinking normally from around the tenth day postoperation. Body weight gain was normal in these rats, though there was a long-term loss of body weight compared to controls. Unoperated rats with food intake yoked to lesioned rats showed identical long-term changes in body weight, suggesting that the changes in body weight of lesioned rats may be a reflection of changes in eating and drinking rather than a disruption of a body weight set-point mechanism. Motor deficits were not found; all rats were able to consume without difficulty saccharin solutions. All lateral hypothalamic lesioned rats failed to respond to dehydrating, dipsogenic of glucoprivic challenges. It is concluded that NMDA is an effective toxin in the rat lateral hypothalamus, sparing ascending dopamine fibres, and that the main effect of such lesions is an impairment in responding to physiological challenges.
AB - The purpose of this study was to determine whether the excitotoxin N-methyl-d-aspartate (NMDA) could be used to make lesions within the lateral hypothalamus and what effect they had on regulatory behaviour. Larger doses of NMDA were effetive in the lateral hypothalmus but tended to spread into adjacent structures; smaller doses made lesions which were contained within the lateral hypothalamus and zona incerta. Lesions which damaged the lateral hypothalamus and surrounding tissue had no effect on the concentration of dopamine (or its metabolites) in the dorsal or ventral striatum. The large lesions, including extrahypothalamic damage, were associated with long-term deficits in lab chow and water intake, but rats with lesions restricted to the lateral hypothalamus made good recoveries, eating and drinking normally from around the tenth day postoperation. Body weight gain was normal in these rats, though there was a long-term loss of body weight compared to controls. Unoperated rats with food intake yoked to lesioned rats showed identical long-term changes in body weight, suggesting that the changes in body weight of lesioned rats may be a reflection of changes in eating and drinking rather than a disruption of a body weight set-point mechanism. Motor deficits were not found; all rats were able to consume without difficulty saccharin solutions. All lateral hypothalamic lesioned rats failed to respond to dehydrating, dipsogenic of glucoprivic challenges. It is concluded that NMDA is an effective toxin in the rat lateral hypothalamus, sparing ascending dopamine fibres, and that the main effect of such lesions is an impairment in responding to physiological challenges.
KW - dopamine
KW - drinking
KW - eating
KW - excitotoxic lesion
KW - lateral hypothalamus
KW - N-methyl-d-aspartate
KW - rats
UR - http://www.scopus.com/inward/record.url?scp=0025221171&partnerID=8YFLogxK
UR - https://link.springer.com/journal/221
U2 - 10.1007/BF00228804
DO - 10.1007/BF00228804
M3 - Article
VL - 82
SP - 628
EP - 636
JO - Experimental Brain Research
JF - Experimental Brain Research
SN - 0014-4819
IS - 3
ER -