Excitation of fluorescence decay using a 265 nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers

Colin D. McGuinness, Alexander M. Macmillan, Kulwinder Sagoo, David McLoskey, David J. S. Birch

Research output: Contribution to journalArticle

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Abstract

The authors describe the characteristics and application of a 265 nm AlGaN light-emitting diode (LED) operated at 1 MHz repetition rate, 1.2 ns pulse duration, 1.32 mu W average power, 2.3 mW peak power, and similar to 12 nm bandwidth. The LED enables the fluorescence decay of weakly emitting phenylalanine to be measured routinely, even in dilute solution. For pH of 6-9.2, the authors find evidence for a biexponential rather than monoexponential decay, providing direct evidence for the presence of phenylalanine rotamers with a photophysics closer to the other two fluorescent amino acids tryrosine and tryptophan than has previously been reported. (c) 2006 American Institute of Physics.
LanguageEnglish
Pages063901
Number of pages3
JournalApplied Physics Letters
Volume89
Issue number6
DOIs
Publication statusPublished - 7 Aug 2006

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phenylalanine
light emitting diodes
fluorescence
tryptophan
decay
excitation
amino acids
repetition
pulse duration
bandwidth
physics

Keywords

  • aluminium compounds
  • gallium compounds
  • light emitting diodes
  • biological techniques

Cite this

McGuinness, Colin D. ; Macmillan, Alexander M. ; Sagoo, Kulwinder ; McLoskey, David ; Birch, David J. S. / Excitation of fluorescence decay using a 265 nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers. In: Applied Physics Letters. 2006 ; Vol. 89, No. 6. pp. 063901.
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Excitation of fluorescence decay using a 265 nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers. / McGuinness, Colin D.; Macmillan, Alexander M.; Sagoo, Kulwinder; McLoskey, David; Birch, David J. S.

In: Applied Physics Letters, Vol. 89, No. 6, 07.08.2006, p. 063901.

Research output: Contribution to journalArticle

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T1 - Excitation of fluorescence decay using a 265 nm pulsed light-emitting diode: Evidence for aqueous phenylalanine rotamers

AU - McGuinness, Colin D.

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AU - Birch, David J. S.

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AB - The authors describe the characteristics and application of a 265 nm AlGaN light-emitting diode (LED) operated at 1 MHz repetition rate, 1.2 ns pulse duration, 1.32 mu W average power, 2.3 mW peak power, and similar to 12 nm bandwidth. The LED enables the fluorescence decay of weakly emitting phenylalanine to be measured routinely, even in dilute solution. For pH of 6-9.2, the authors find evidence for a biexponential rather than monoexponential decay, providing direct evidence for the presence of phenylalanine rotamers with a photophysics closer to the other two fluorescent amino acids tryrosine and tryptophan than has previously been reported. (c) 2006 American Institute of Physics.

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