Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940

Charles W. Knapp, Jan Dolfing, Phillip A.I. Ehlert, David W. Graham

Research output: Contribution to journalArticle

411 Citations (Scopus)

Abstract

Mass production and use of antibiotics and antimicrobials in medicine and agriculture have existed for over 60 years, and has substantially benefited public health and agricultural productivity throughout the world. However, there is growing evidence that resistance to antibiotics (AR) is increasing both in benign and pathogenic bacteria, posing an emerging threat to public and environmental health in the future. Although evidence has existed for years from clinical data of increasing AR, almost no quantitative environmental data exist that span increased industrial antibiotic production in the 1950s to the present; i.e., data that might delineate trends in AR potentially valuable for epidemiological studies. To address this critical knowledge gap, we speculated that AR levels might be apparent in historic soil archives as evidenced by antibiotic resistance gene (ARG) abundances over time. Accordingly, DNA was extracted from five long-term soil-series from different locations in The Netherlands that spanned 1940 to 2008, and 16S rRNA gene and 18 ARG abundances from different major antibiotic classes were quantified. Results show that ARG from all classes of antibiotics tested have significantly increased since 1940, but especially within the tetracyclines, with some individual ARG being >15 times more abundant now than in the 1970s. This is noteworthy because waste management procedures have broadly improved and stricter rules on nontherapeutic antibiotic use in agriculture are being promulgated. Although these data are local to The Netherlands, they suggest basal environmental levels of ARG still might be increasing, which has implications to similar locations around the world.
Original languageEnglish
Pages (from-to)580-587
Number of pages8
JournalEnvironmental Science and Technology
Volume44
Issue number2
Early online date23 Dec 2009
DOIs
Publication statusPublished - 7 Jan 2010

Fingerprint

antibiotic resistance
antibiotics
Genes
Anti-Bacterial Agents
Soils
gene
soil
public health
agriculture
Agriculture
medicine
waste management
Tetracyclines
DNA
productivity
bacterium
Public health
Waste management
Medicine
Bacteria

Keywords

  • antibiotic resistance
  • antibiotics
  • gene abundances
  • soils
  • pathogenic bacteria
  • waste management

Cite this

Knapp, Charles W. ; Dolfing, Jan ; Ehlert, Phillip A.I. ; Graham, David W. / Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940. In: Environmental Science and Technology. 2010 ; Vol. 44, No. 2. pp. 580-587.
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Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940. / Knapp, Charles W.; Dolfing, Jan; Ehlert, Phillip A.I.; Graham, David W.

In: Environmental Science and Technology, Vol. 44, No. 2, 07.01.2010, p. 580-587.

Research output: Contribution to journalArticle

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